CTSI Cores Search

CTSI Cores Search

Many outstanding core facilities and specialized research laboratories exist at CTSI partner institutions. These facilities provide highly specialized services, equipment, and staff.

CTSI is committed to increasing awareness of these resources to expedite clinical and translational research. Therefore, the Cores Search tool has been created in order to inventory research resources for CTSI research teams.

These core facilities and specialized research laboratories are owned, supported, and managed by the institutions that comprise the CTSI of Southeast Wisconsin. Most of these cores are publicly available. However, some are required to limit access. Policies regarding services, access, fees and prioritization vary by core and institution. Please contact a person listed on the page of the facility/service of interest for more information.

FILTER:
LocationLocationNameDescriptionLinkCTSI Core/ServiceFree Core Access
LocationLocationNameDescriptionLink
BloodCenter of WisconsinbcwBIOPHYSICS CORE More InfoThe Biophysics Core consists of one system, the BIACore 3000.

The Biophysics Core at BloodCenter of Wisconsin’s Blood Research Institute provides the data gathering services of this system and the dedicated computers to all its investigators and outside users as capacity permits.

The BIACore 3000 uses the optical phenomenon of surface plasmon resonance (SPR) to monitor the formation and dissociation of bio-molecular complexes on a sensor surface as the interaction occurs. SPR is a non-invasive technique, which measures the mass concentration of biomolecules in close proximity to a specially prepared surface. By covalently attaching one molecule (the ligand) to the surface of a chip that forms one wall of a flow cell, then exposing it, under the conditions of continuous flow, to a second molecule (the analyte) in solution, interactive measurements can be made that are essentially independent of the nature of the biomolecule without the labeling of the interactants.
NoNo
BloodCenter of WisconsinbcwFLOW CYTOMETRY CORE More InfoThe Flow Cytometry Core contains 4 Becton Dickinson cytometer systems.

Instrumentation and Applications
BD LSRII Flow Cytometer
Capable of 10 color and 12 parameter acquisition
4 laser system includes blue 488nm, red 640nm, yellow-green 561nm and UV 355nm
BD HTS can be used on this instrument. Capable of reading 96 well and 384 well microtiter plates

BD LSRII Special Order System
Capable of 12 color and 14 parameter acquisition
4 laser system includes blue 488nm, red 640nm, green 532nm and violet 405nm
BD HTS can be used on this instrument. Capable of reading 96 well and 384 well microtiter plates
BD FACSAria Cell Sorter
Capable of 10 color and 12 parameter acquisition
4 laser system includes blue 488nm, red 633nm, green 532nm and violet 407nm

Accuri C6
Capable of 4 color and 6 parameter acquisition
2 laser system includes blue 488nm and red 633nm

The core provides data analysis with the following software:
Analysis workstations are available as well as training for flow core users.

FlowJo
FCS Express
FACSDiva
CFlow Plus

Miltenyi Biotec Magnetic Cell Separation Workstation
This workstation consists of a stand and 4 separation magnets that can be reserved for use. The usually vast initial cell population can be much reduced and greatly enriched for the target cell, with little or no added abuse to the cells. This cuts sorting time and usually increases viability.
NoNo
BloodCenter of WisconsinbcwHISTOLOGY CORE More InfoThe Histology Core provides services for tissue sectioning, preparation and staining. This core is operated jointly with the Physiology Department of the Medical College of Wisconsin where it is physically located. Core staff provide services in tissue processing, embedding, sectioning, and basic staining procedures. A cryostat is available for frozen section preparation.

Instrumentation and application
Automated tissue processor
This processor is used for paraffin sections. Tissues are preferred in 10% neutral buffered formalin.

Sakura automated stainer
The stainer can process 40 slides per run, hematoxylin and eosin (H & E). Dewaxing and hydrating services are available. These are useful when the user’s lab wants to perform its own specialized staining procedures.

Cryostat
The cryostat is used for frozen sections. OCT mounting media is available.

Manual staining
Stains available include trichrome, iron, periodic acid schiff (PAS) and others.
NoNo
BloodCenter of WisconsinbcwHYBRIDOMA CORE LAB More InfoThe Hybridoma Core Lab provides services and products related to the production of monoclonal antibodies. Basic procedures include mouse immunization, with immunogen provided by requestor. Subsequent boost and bleeds are done to check antibody titer in the animal. Spleen cells are fused with a non immunoglobulin-secreting mouse myeloma cell line.

The hybridomas are cultured in standard selection medium. These hybridomas are screened by a variety of methods by the requesting lab, or by the Hybridoma Core by ELISA. Up to 10 are cloned by limiting dilution and screened again. Once a stable cell line is produced and its isotype is determined, the core lab can produce ascites in vivo, or use in vitro methods of antibody production.

Cells are frozen at each stage of production and are kept in two locations. Generally, fusions are done with Balb/c mice. The Hybridoma Core lab offers related services as time and demand permits.

The Hybridoma Core also provides other tissue culture services. Endothelial cells from human umbilical vein are available upon request. Secondary passage and higher are kept in stock and can be ready with a week’s notice. Primary cultures require more advanced notice.

Core Policies and Services
Ascites production comes from in-house hybridoma or ATCC cell lines. If there is a sufficient supply, 3-15 ml of ascites can be provided from freezer inventory within 24 hours. If the ascites need to be produced in mice or in vitro, the wait time can be 3 weeks to 3 months depending on demand.

New fusions are on a first come, first served basis. Preference is given to BRI researchers. Generally, the requesting lab needs to provide about 1 mg of purified protein or peptide coupled to a carrier protein. The immunization period is typically 3 to 4 months. Screening, cloning, subcloning and freezing of cells can take an additional 3 to 6 months. All screening methods are to be worked out prior to fusion date. A technologist and the Scientific Director meet with each requesting lab to discuss the process and screening approach.
NoNo
BloodCenter of WisconsinbcwIMAGING CORE More InfoThe Imaging Core at BloodCenter’s Blood Research Institute (BRI) provides services in microscopy and analysis of two-dimensional images. Staff assists investigators and technologists through training for the use of microscopes and software packages that digitize images.

Instrumentation and Applications
Zeiss Lumar .V12, Dissecting scope
The Lumar is used for specimen dissection and analysis. It uses a 1.6X or 0.8X objective allowing for high resolution of specimens and great working distance. Fluorescence filter sets allow for the excitation of GFP, CFP and Rhodamine stained samples. Images can be acquired using a 5 megapixel Zeiss AxioCam MRc 5 camera. Perform further image analysis using Zeiss AxioVision 4.5 software.

NikonTE200 with Hoffman modulation, Inverted
Used for live cell cultures and prepared mounted slides. This microscope is outfitted with PLAN Fluor objectives 2X, 4X, 10X, 20X, 40X, and 60X. Fluorescence images can be acquired using filter sets for Hoeschst, DAPI, FITC, Texas Red, and Cy5. Images can be taken with either a Diagnostic Instruments Spot RT color camera using spot advanced software or a Photometrics CoolSNAP ES camera using Metamorph 6.1 software.

Nikon E600, Upright
Used for analysis of prepared mounted slides. This microscope is outfitted with Plan Fluor objectives ranging from 4X -100X. Images can be acquired using a Diagnostic Instruments Spot insight color camera using spot advanced software.

Zeiss AxioScope, Upright
Used for analysis of prepared mounted slides. This microscope uses high resolution Plan Neofluar optics ranging from 10X -100X. Fluorescence images can be acquired with filters sets exciting for DAPI, FITC Tex Red, Dual, and Rhodamine. Acquire images with a Photometric SenSys camera using Metamorph 6.1 software.

Metamorph imaging software
This software is used to measure and quantify different aspects of images. For example, sizes and numbers of cells, circumference of structures, and areas of portions of interest can be determined using this high throughput system.
NoNo
BloodCenter of WisconsinbcwMOLECULAR BIOLOGY CORE More InfoThe Molecular Biology Core Laboratory offers automated DNA sequencing services. In addition, the Core houses and maintains a real-time PCR detection system, and a suspension array system.

Instrumentation and Applications
ABI 3100×1 Genetic Analyzer
Automated sequencing is performed on an ABI 3100×1 Genetic Analyzer. This capillary-based platform allows for processing of up to 96 samples in an 18-hours. Samples are run within 1 to 2 days and yield a high-quality read length of up to 600+ base pairs.

Applied Biosystems 7500 Real-Time PCR System
This 7500 RT PCR System uses a 5-color platform calibrated for FAM/SYBR Green I, VIC/JOE, NED/TAMRA/Cy3, ROX/Texas Red, and Cy5 dyes.

Bio-Plex Array Reader and Microplate Platform
The flow-based, dual-laser array reader classifies each bead and its associated assay, and quantitates the amount of analyte captured. The microplate platform permits automated processing of samples from 96-well microplates in approximately 30 minutes. Using as little as 12 µl of serum or other biological sample per multiplex assay, this bioassay system allows for simultaneous detection and quantitation of up to 100 different analytes in a single well.

DNA Sequencing Services
Generally, users perform their own cycle sequencing reactions using ABI’s Big Dye Terminator chemistry (BRI internal users can purchase their sequencing reagents from the Core). The turnaround time is typically 1 to 2 working days after sample submission. For a nominal fee, users may request complete sample sequencing reaction preparation. Templates and primers are submitted separately in water. Users who opt for this service should contact us prior to sample submission to ensure proper processing of your samples.

Quantitative (real time) PCR Policies
Interested users should sign up before using the instrument. Sign-up is done on Outlook Public Folders under Core Schedules folder.
NoNo
BloodCenter of WisconsinbcwPROTEIN CHEMISTRY CORE More InfoThe Protein Chemistry Core Lab of the Blood Research Institute was established in 1988 and offers custom peptide synthesis and purification utilizing both natural and non-natural amino acids. Modifications such as biotinylation, fluorescent labeling, conjugation to carrier proteins, cyclization and incorporation of stable isotopes are routinely done. In addition, the Core Lab now supports isoelectric focusing, 2-D gel electrophoresis and digital gel imaging. The Core Lab has been a member of the Association for Biomolecular Resource Facilities since 1995.

Instrumentation and Applications
Peptide synthesis
The CEM LIBERTY1 is a microwave-assisted single channel instrument with UV monitor that generates high purity peptides with fast cycle times. The synthesis scales are from 0.05mmol to 3mmol. Peptides are synthesized using FMOC chemistry on solid supports or resins. Many modifications are possible including N and C terminal labeling or capping, side chain modifications, backbone modifications and stable isotope labeling. Peptides are purified by reverse-phase HPLC on a Beckman System Gold with a UV detector. Peptide masses are verified by MALDI-TOF mass spectral analysis. Synthetic peptides are delivered as dry lyophilized powders. Yield is based on scale and sequence.

Useful Links
Technical Resource Library for Peptide Synthesis Sigma-Aldrich
Peptide and Organic Synthesis Technical Resources EMD Millipore

Isoelectric Focusing / 2-D Gel Electrophoresis / Electroblotting
The technique of two-dimensional electrophoresis involves separating proteins in the first dimension according to charge (isoelectric focusing), followed by MW separation in the second dimension by SDS-PAGE. Isoelectric focusing is performed using a Biorad Protean IEF cell. This system uses immobile pH gradients (IPG) gels adhered to a plastic back.

The proteins are then visualized by staining the gel with Coomassie stain, silver stain or fluorescent dyes. This two dimensional array will produce spots that correspond to single protein species in the sample. Using this technique, different proteins can be separated and information such as pI, molecular weight and protein abundance can be determined. 2D-gels can be electroblotted to PVDF or nitrocellulose membranes for further analysis.

Digital Imaging
The Licor Odyssey Imaging system uses direct infrared detection that provides accurate quantification, sensitivity and a wide linear range. Two IR channels can be read simultaneously to probe two separate targets or to increase quantification accuracy by using the second channel for normalization. The Odyssesy is suitable for gels, membranes and glass slides.

The GE Healthcare Typhoon Trio is a variable mode imager that can be used for the acquisition of fluorescent or chemiluminescent data. The scanner is capable of imaging gel sandwiches, agarose and polyacrylamide gels, membranes, microplates and microarrays. Multiple dyes can be analyzed per sample. After acquisition, the images can be quantitated using ImageQuant TL from GE.

Core Policies and Services
The average turnaround time for standard peptide synthesis is two weeks, longer if modification or non standard amino acids are required. The peptide yield depends on the length and the purity required. The user will be advised if the project does not seem feasible or if low yields are expected.

Training is required before operating the Protean IEF, the LICOR Odyssey or the Typhoon Trio. Online calendars are maintained for the Protean IEF, Typhoon, Odyssey. Paper calendars are maintained for the AKTA and the Agilent. Users must sign up in advance to use these instruments.
NoNo
BloodCenter of WisconsinbcwVIRAL VECTOR CORE More InfoThe Viral Vector Core is shared between the Blood Research Institute and the Medical College of Wisconsin. It is located in the east wing of the BRI, room 2025. Vector systems used by the core include those based on lentivirus, retrovirus, adenovirus, and adeno-associated virus. This core provides services in the areas of vector design, gene silencing and protein expression, including construction, amplification, purification and titration. Additional services include cloning, mutagenesis, and plasmid DNA preparation.

LOCATION

Blood Research Institute, 8733 Watertown Plank Road

SERVICES

Lentiviral/Retroviral Vector Production:
Small-scale (plates) and large-scale (roller bottles)

Lentiviral/Retroviral Vector Titration:
Flow cytometry assay, integration qPCR, replication competence testing

Adenoviral Vector Production:
Recombination and transfection, small and large-scale amplification, purification

Adenoviral Vector Titration:
Flow cytometry based antibody assay

Adeno-associated Viral Vector Production:
Small and large-scale production and purification

Other:
Vector construction, site-directed mutagenesis, plasmid prep

*Various viral vectors expressing specific reporter genes such as GFP, YFP, mCherry and Cerulean can be purchased for testing and generation of preliminary data
NoNo
Children's Hospital of WisconsinchwHISTOLOGY CORE More InfoThe CRI Histology Core offers a broad range of high quality histological, immunohistochemical, and other related services on a fee-for-service basis to investigators from the Children’s Hospital of Wisconsin Research Institute, the Medical College of Wisconsin, and other institutions.

This full-service Histology Core facility, operated in conjunction with the Pediatric Pathology Division of the Department of Pathology, offers internal and external users the following services:

Frozen and fixed tissue embedding
Processing
Cryostat and microtome sectioning
Routine H&E staining
Specialized histological staining
Immunohistochemical and immunofluorescent staining.
Optimization of immunostaining protocols using investigator-provided antibodies
Training in histological and immunohistochemical techniques
4 ASCP-certified histotechnologists and an American Board of Pathology-certified pathologist provide quality control.
Preparation of slides for laser capture microdissection
Preparation of tissue microarrays (in collaboration with Pediatric Biobank staff)
Close coordination of staining with imaging services provided by the physically adjacent CRI Imaging Core

Equipment/Software

Equipment/Software
Two Immunohistochemistry automated stainers (Leica – Bond Max Automated Stainer and DAKO Autostainer Plus)
Dako Artisan Special Stainer
Two Sakura Tissue-Tek VIP5 and one VIP6 tissue processors
Sakura Tissue-Tek Embedding Center Tec 5
Leica CM1850 UV Cryostat
Two Microm HM355S motorized rotary microtomes
Robotic routine stainers (Sakura Prisma)
Automated Coverslipper (Sakura Glas)
Leica IP S slide labeler
Leica Vibratome HM650 and Cooling Unit
NoNo
Children's Hospital of WisconsinchwIMAGING CORE More InfoWe work with investigators at Children’s Hospital of Wisconsin Research Institute and other collaborating institutions to provide fee-based assisted and unassisted access to a variety of state-of-the-art microscopic imaging systems.

Operated by the Pediatric Pathology Division at the Medical College of Wisconsin and Children’s Research Institute, our microscopy-based Imaging Core is open to all investigators at collaborating institutions.

Access to microscopic and imaging systems is controlled, monitored and protected by sign-up sheets, card reader entry and individualized computer account sign-on. Unassisted use of Imaging Core equipment requires prescribed training, demonstration of competency and approval by the core director. Investigators already affiliated with Children’s Research Institute can find more information about the Imaging Core’s equipment, fees and policies on the Children’s Hospital of Wisconsin intranet.

TECHNOLOGY & RESOURCES

Zeiss Axioimager Z1
Zeiss Axiovert 200M
Zeiss P.A.L.M. Microbeam III
Zeiss LSM510 confocal
Zeiss LSM510 META NLO multiphoton
NoNo
Children's Hospital of WisconsinchwMCW CANCER CENTER AND CHILDREN’S RESEARCH INSTITUTE (CRI) SHARED FACILITY More InfoThe Medical College of Wisconsin Cancer Center (MCW-CC) and Children’s Research Institute (CRI) shared Flow Cytometry Facility is an advanced technology facility primarily serving investigators at the Medical College and the Children’s Research Institute. The facility provides secondary support to on-campus colleagues at the Blood Research Institute, as well as collaborators off campus at the University of Wisconsin-Milwaukee, Marquette University and the Marshfield Clinic.

The first MCW-CC facility was established in 2000 with support from sources including a NIH Shared Instrumentation Grant, MCW Corporate Support, and the MCW-CC. The CRI facility was established in 2005 with support from the Children’s Foundation. In 2013, the two facilities merged in order to contain costs while improving access and service.

Now located on the 5th floor of the MACC Fund Research Center, the reorganized facility provides services to ~60 investigators campus-wide, for a combined total of more than 140 investigators served since 2000. The Children’s Research Institute and the Cancer Center share administrative oversight of the facility.

Services
In addition to instrumentation, training, and cell sorting services, the facility also provides consultation to investigators regarding experiment design and protocol development based on instrument capabilities. The facility can advise antibody and fluorochrome selection based on targets of interest, appropriate compensation controls when needed, and provide software support for data analysis.
Equipment/Software
FACS Calibur
FACS Aria SOS
LSR II
FlowJo
NoNo
Children's Hospital of WisconsinchwNUCLEIC ACID EXTRACTION CORE More InfoThe Nucleic Acid Extraction Core provides investigators with fee-based services for DNA extraction using either manual and/or automated methods for the following sample types: Whole blood in small, medium or large volumes (400ul, 1-5mL, and 5-10mL); Tissue (fresh, frozen, and formalin fixed paraffin embedded tissue); and Buccal swabs.

LOCATION

Children’s Research Institute, C4210

TECHNOLOGY & RESOURCES

Promega Maxwell 16
NoNo
Children's Hospital of WisconsinchwPEDIATRIC & ADOLESCENT MEDICINE TRANSLATIONAL RESEARCH UNIT More InfoLocated on the fourth floor of Children’s Hospital of Wisconsin, the Pediatric Translational Research Unit (P-TRU) is a resource for all clinical investigators to perform the translational aspects of their behavioral, clinical and/or observation studies. The P-TRU services all ages from birth through geriatrics. Most of the research studies are specific to genetic congenital anomalies but range for all populations.

SERVICES

The P-TRU team is available to assist with a variety of services:

Study management: from budgeting, planning, and regulatory to implementation
Our health unit coordinator, also a trained phlebotomist, is available to assist investigators with specimen processing
Friendly research nurses are available to assist investigators with research specific needs across multiple disciplines
Team members provide assistance with scattered bed placements, inpatient/outpatient research, behavioral health, interviews, lab services and qualitative health sciences
Grant development resources and assistance with protocol implementation are also provided
The P-TRU research team provides a unique combination of research and quality care while enhancing the quality of the experience for research participants
EPIC support for research specific eduation, order entry, and billing
The P-TRU offers five exam suites (two of which can accommodate large families) and also provides additional space for investigators to conduct interviews and obtain informed consent.

Multiple refrigerators
A -80˚ freezer
An fMRI
DEXA Scanner
Bod Pod
Pea Pod (which is mobile and allows for flexibility in location)
YesNo
Children's Hospital of WisconsinchwPEDIATRIC BIOBANK & ANALYTICAL TISSUE CORE More InfoThe CRI Pediatric BioBank & Analytical Tissue Core, a research-supportive facility, directed by the Division of Pediatric Pathology with major support from the MACC Fund, is housed on the 4th floor of the TBRC/CRI building and provides quality-controlled banking of human pediatric tissue, blood, and urine samples for use by investigators at MCW and its affiliated institutions.

Additionally, the core offers tissue analytical services, such as nucleic acid extraction (in collaboration with the CRI Nucleic Acid Extraction Core) from solid tissues (fresh, frozen, or formalin-fixed-paraffin-embedded) as well as from fresh or frozen blood, high-resolution digital microscope slide scanning, laser scanning cytometry, and image analysis for quantification of parameters in brightfield and fluorescent images.

Equipment/Software
Hamamatsu Nanozoomer slide scanner
Laser scanning cytometer
Visiopharm image analysis software
Promega Maxwell DNA extraction system
NoNo
Children's Hospital of WisconsinchwPEDIATRIC ECHOCARDIOGRAPHY CORE LABORATORY More InfoThe Pediatric Echocardiography Core Lab housed in Children’s Hospital of Wisconsin was established in 2005 and has functioned as a resource for both internal and external projects that require performance of echocardiograms and/or analysis of echocardiographic data. The lab has received multiple NIH sub-contract grants from the Pediatric Heart Network (PHN) to act as an echo core lab for several large, multi-institutional trials: the Single Ventricle Reconstruction Trial, the Single Ventricle Reconstruction Extension Trial, and the Pediatric Echocardiography Database Z-score Project. The core lab will have analyzed over 7000 echocardiograms for these trials as well as collating and managing all of the echo data.

In addition to the work with the NIH trials, the echo core lab has supported multiple industry and investigator-initiated research projects involving echocardiography. The echo core lab is located on the 2nd floor of the west tower of Children’s Hospital in the Herma Heart Center and provides services to investigators for both the adult and pediatric TRUs of the CTSI. We have trained staff with expertise in congenital heart disease and pediatric echocardiography (including 2D, 3D, and myocardial deformation imaging).

LOCATION

Children’s Hospital, Herma Heart Center

EQUIPMENT/HARDWARE

TomTec Workstations (3): HP Z420 with Xeon E5-1620 3.6 GHz (quad core), 10 MB cache per processor, 8 GB RAM, 500 GB hard drive, nVidia Quadro K2000 with 2 GB SDRAM video, DVD +/- RW drive with DVD RAM, Gigabit LAN networking, running Windows 7 64-bit operating system.
SOFTWARE

Image Arena TM 4.6 (Image analysis software – TomTec Imaging Systems, Germany): Diagnostic and report management system specifically designed for 2D/3D echo image review, archiving, and reporting with password-protected access
2D Cardiac Performance Analysis© software (ver. 1.2.0.27; TomTec Imaging Systems, Germany): Myocardial mechanics analysis tool that can analyze images from any US vendor (3 licensed PC’s)
PASW 18: (Predictive Analytic Software): Statistical Analysis Software
RESOURCES

Sequoia 512 Ultrasound Machine (4, 8, and 10 MHz 2D imaging probes)
Accuson SC2000 Ultrasound Machine (8 and 4 MHz 2D imaging and 4 MHz 3D imaging probes)
Phillips IE33 Ultrasound Machine (12, 8, 5 MHz 2D imaging and 7 and 3 MHz 3D imaging probes)
SERVICES

Performance of 2D and 3D echo imaging studies (pediatric or adult)
Protocol development/consultation
Data collection tool development
Data collection and management
Technical image review and analysis
Data collation
Physician review*
Investigator consults for planning and developing echo components of research protocols
Writing of echo components of research protocols, abstracts and manuscripts
Screening subjects for study eligibility
Data cleaning and basic descriptive statistical analysis*
*These services and fee schedules are discussed individually for each study

TRAINING

Echo image analysis training for students, nurses, research coordinators, residents, fellows and physicians.
NoNo
Froedtert HospitalfhADULT ECHOCARDIOGRAPHY CORE LABORATORY More InfoThe Adult Echocardiography Core provides high-quality cardiovascular imaging along with quantitative and qualitative imaging analysis for cardiovascular clinical trials and investigator initiated protocols. The Core faculty and staff offers expertise, materials, consultation, performs scanning and analysis on a fee for service basis.

The Core serves Investigators affiliated with the Medical College of Wisconsin. We provide consultation on image acquisition and analysis based upon American Society of Echocardiography (ASE) recommendations for the performance and evaluation of a comprehensive transthoracic echocardiogram, use of echocardiography in clinical trials and employ the ASE Standards for Echocardiography Core Laboratories.

Successful and timely scanning and data analysis requires a concerted, collaborative effort of the Investigator and the Core staff in all stages of the study. Investigators planning on utilizing Core services are therefore encouraged to contact the staff early in the planning stages of the study.
YesNo
Froedtert HospitalfhADULT TRANSLATIONAL RESEARCH UNIT More InfoScientists at the Medical College of Wisconsin and at any of the CTSI partner institutions who have an approved protocol can utilize the TRU facilities. We provide support services for a diverse array of research studies, which involve pulmonary, cardiovascular, chronic kidney, neurological (Alzheimer’s), metabolic, sickle cell, genetics and rare diseases to include a few.

The Adult TRU is located in close proximity to the main entrance of the Pavilion building of Froedtert Hospital. The unit is able to provide a wide array of basic clinical research services including nursing support, dedicated nursing time, use of exam rooms and equipment, laboratory sample preparation and consultations. Special arrangements can be made for studies requiring TRU support in a hospital setting.

SERVICES - The A-TRU team is available to assist with a variety of services including : Research nursing support and dedicated nursing time, Laboratory sample preparation and processing, Assistance with inpatient studies, Glucose Analysis, and Noninvasive BP Monitoring.
YesNo
Froedtert HospitalfhBIONUTRITION AND BODY COMPOSITION LAB More InfoThe Clinical and Translational Science Institute’s Bionutrition and Body Composition Lab is based out of the Adult Translational Research Unit (ATRU) and provides services to researchers from all CTSI affiliated institutions and community organizations.

Researchers and community health initiatives find that body composition and nutrition play an important role in promoting health and the treatment of disease. Even if nutrition and body composition are not a primary focus, an estimate on body composition and nutrient intake is often desired due to the influence both have on human physiology and public health determinates.

OVERVIEW

The CTSI Bionutritionist assists with planning, developing, implementing, and evaluating the nutrition and body composition components of research protocols and community programs. The ATRU houses a metabolic kitchen dedicated to providing general or protocol specific meals. It also has a body composition lab where whole body DXA scans, anthropometric measurements, bioelectrical impedance analysis, and energy assessment take place.
YesNo
Froedtert HospitalfhCLINICAL AND TRANSLATIONAL RESEARCH CORE LAB (CTRL) HISTOLOGY More InfoThe Clinical and Translational Research Core Lab (CTRL) combines two major components in one lab: histopathology and molecular pathology. The CTRL provides basic histopathology, research immunohistochemistry, tissue microarray, laser capture microdissection, and molecular biology services to researchers at the Medical College of Wisconsin and within the surrounding academic community.

Histopathology services include: tissue processing, embedding, and cutting both paraffin-embedded and frozen tissue. The CTRL performs standard stains, immunohistochemistry, antibody workup, and in situ hybridization. Tissue microarrays are constructed using a Veridiam VT110C. Digital imaging includes the ACIS III (DAKO), Visiopharm, and Nanozoomer (Hamamatsu). Specialized software is available to image and analyze tissue microarrays, and to manage and store array data.

The histology activities are performed by an HT (ASCP) certified histology technologist with twenty years of histology experience.

Histology Core Lab Services

Gross description and analysis of tissue specimens
Gross dissection of specimens (MOPEC)
Slide printer with barcoding (ThermoSlidemate)
Cassette printer with barcoding (ThermoPrintmate)
Tissue processing (Sakura VIP6)
Tissue paraffin embedding (Sakura TEK6)
Tissue sectioning to make slides (Thermo HM 355S)
Frozen sections (Thermo HM 525)
Slide staining (Leica Autostainer)
Immunohistochemical analysis (DAKO Autostainer XL)
Immunofluorescence (Olympus BX61)
Special staining
Tissue microarray construction (Veridiam)
Quantitative image analysis (DAKO ACIS III)
Laser capture microscopy (Arcturus)
Pathological interpretation
Tumor Identification

Equipment/Software

MOPEC Gross dissection of specimens

ThermoSlidemate slide printer with barcoding

ThermoPrintmate cassette printer with barcoding

Sakura VIP6 tissue processing

Sakura TEK6 tissue paraffin embedding

Thermo HM 355S tissue sectioning to make slides

Thermo HM 525 frozen sections

Leica Autostainer slide staining

DAKO Autostainer XL immunohistochemical analysis

Olympus BX61 immunofluorescence

Veridiam Tissue microarray construction

DAKO ACIS III quantitative image analysis

Arcturus laser capture microscopy

Leica Cytovision Software for FISH Analysis
NoNo
Froedtert HospitalfhCLINICAL AND TRANSLATIONAL RESEARCH CORE LAB (CTRL) MOLECULAR SERVICES More InfoThe Clinical and Translational Research Core Lab (CTRL) combines two major components in one lab: histopathology and molecular pathology. The CTRL provides basic histopathology, research immunohistochemistry, tissue microarray, laser capture microdissection, and molecular biology services to researchers at the Medical College of Wisconsin and within the surrounding academic community.

Molecular Diagnostic Services

The CTRL offers clinical molecular diagnostic testing for routine patient care, clinical trials, and research. Molecular services include: nucleic acid extraction from tissues (fresh, frozen, blood, formalin fixed paraffin embedded), nucleic acid quantification, PCR, real time PCR, quantitative PCR, dideoxy sequencing, massively parallel sequencing, capillary electrophoresis, gel electrophoresis, digital electrophoresis, and microarray analysis (Agilent format).

Molecular Core Lab Services

Nucleic acid purification (Qiagen BioRobot EZ1)
Nucleic acid quantification (Nanodrop; Qbit)
Agilent Bioanalyzer
PCR (ABI Veriti)
Real time PCR (Lightcycler; ABI 7500)
Reverse transcriptase PCR
Quantitative PCR (Lightcycler; ABI 7500)
Dideoxy sequencing (ABI 3500)
Ion Torrent PGM
DNA fragment analysis (ABI 3500)
DNA microarray analysis (Innopsys Scanner)
Ozone-free working environment (SciGene)
Gene expression microarray analysis (Innopsys)
Gel electrophoresis (Agarose, acrylamide)
Fluorescence in situ hybridization (FISH)

Equipment/Software

Qiagen BioRobot EZ1 nucleic acid purification

Nanodrop; Qbit nucleic acid quantification

Agilent Bioanalyzer

ABI Veriti PCR

Lightcycler2.0

ABI 3500 DNA fragment analysis and dideoxy sequencing

Ion Torrent PGM

Innopsys Scanner DNA/RNA microarray analysis

SciGene ozone-free working environment

Agarose, acrylamide gel electrophoresis

ABI 7500 Quantitative PCR
NoNo
Froedtert Hospitalfhi2b2 COHORT DISCOVERY TOOL More InfoThe i2b2 Cohort Discovery Tool provides an easy to use, self-service way for the Medical College of Wisconsin researchers to query the i2b2 Clinical & Translational Research Informatics Data Warehouse (CTRI-CRDW) to answer the question “Does the i2b2 CTRI-CRDW contain a cohort of patients with these characteristics?”. Search criteria can include patient demographics, ICD coded diagnoses, ICD and CPT coded procedures, laboratory test results, inpatient pharmacy orders and text within clinical documents, including surgical pathology reports and radiology reports.

In response to a query, the Cohort Discovery Tool returns the approximate number of patients matching the search criteria. No patient identifiers or clinical data is revealed. Researchers can store their cohort searches online and later meet with staff from the Medical College of Wisconsin Clinical & Translational Research Informatics team to discuss how they might review and extract detailed clinical data on patient cohorts for research purposes. Release of data from the i2b2 CTRI-CRDW for research purposes requires Medical College of Wisconsin IRB approval.
YesNo
Froedtert HospitalfhIRB NAVIGATOR More InfoInvestigators that have received an award from CTSI and require IRB approval for their study can enjoy the assistance of personalized IRB navigation: guidance through eBridge prior to submission, track protocol’s progress as it moves through the review process and track and troubleshoot delays to promote successful completion.

The IRB Navigator is a research service offered to investigators who have received an award from CTSI and require IRB approval for their study. The IRB Navigator provides investigators with assistance through the approval process and helps to ensure the process runs smoothly. Prior to, during and after the application is submitted through eBridge, the IRB Navigator can monitor it and troubleshoot to help advance the application to a successful completion. If there are areas that need further explanation prior to the application going to the IRB committee, the IRB Navigator acts as a liaison between the investigator and the IRB Coordinator to assist in providing clarification so the appropriate information is presented to reviewers and committee chair. After a study is approved, the IRB Navigator can assist with amendments and potentially reportable events.
YesNo
Froedtert HospitalfhLYMPHOCYTE PROPAGATION LABORATORY (LPL) More InfoThe Medical College of Wisconsin Cancer Center Lymphocyte Propagation Laboratory (LPL) is part of the Blood and Marrow Transplantation Program of the Medical College of Wisconsin. This laboratory provides cellular therapy product processing for human clinical trials. The laboratory primarily focuses on cellular therapy to improve the outcome of blood and marrow transplantation and the use of immunotherapy to combat human cancer.

The laboratory is CAP accredited, CLIA certified and is accredited by the Foundation for the Accreditation of Cellular Therapy (FACT) for the testing and processing activities that are performed.

Services include:

Consultation during early and mid-phase development
Identification of acceptable reagent and material sources
Assistance to prepare for FDA pre-IND meeting
Scale-up and/or transition to clinical grade product
Assist with writing of the chemistry, manufacturing, and controls (CMC) section of IND Application
Preparation of FDA compliant SOPs, workforms/batch records, and reports for product preparation and testing
Assist with the selection and validation of assays to ensure product safety, purity, and potency during phase I and II.
Production of products for clinical use and product storage
Release testing
Shipment to external sites
Prep and delivery of products for infusion
Post infusion immune monitoring with Flow Cytometry Shared Resource

Equipment/Software

CliniMACS Cell Separation Device X 2

CliniMACS Prodigy Cell Culture and Separation Device

BD Canto II Flow Cytometer

Top Count

LN2 Controlled Rate Freezers and Storage Tanks

Cobe 2991 Cell Washers x 2

Biological Safety Cabinets

Inverted and standard Microscopes

CO2 Incubators with Hepa Filtration

Sterile Connecting Devices and tubing sealers

Table Top and Floor Model Centrifuges X 4
NoNo
Froedtert HospitalfhOFFICE OF CLINICAL RESEARCH & INNOVATIVE CARE COMPLIANCE (OCRICC) More InfoEven with IRB approval, a project requiring use of any FH resource cannot begin activities without FH final Administrative Approval granted though OCRICC.

Your project must be reviewed by and receive FH Administrative Approval through the FH Office of Clinical Research & Innovative Care Compliance (OCRICC) if you plan to conduct your research at any Froedtert Health (FH) System entity (Froedtert Hospital, Community Memorial Hospital, St. Joseph West Bend, or any of our clinics), and/or utilize any FH resource for your research. These resources include, but not limited to, the following:

Cinical services (i.e., radiology imaging)
Surgical/interventional services
Staff time
Equipment
Space (i.e., inpatient, FH clinics, FH ancillary services, etc.)
Drugs
Devices
Lab services (except for services provided by Dynacare that are set up as external clients)
Specimens, including “discard” tissue from any FH or OR procedure area, and/or
Patient Health Information (PHI)
If you are unsure whether the resources involved in your project are FH resources, or if you are unclear exactly what resources you will need for your project, please contact OCRICC prior to your IRB submission.

OCRICC recommends that you contact them no later than submission to the IRB.
NoNo
Froedtert HospitalfhREDCap More InfoREDCap is a mature, secure web application for building and managing online surveys and databases. While REDCap can be used to collect virtually any type of data, it is specifically geared to support data capture for research studies. The REDCap Consortium is composed of 1,436 active institutional partners in 90 countries who utilize and support REDCap in various ways.YesNo
Froedtert HospitalfhRESEARCH PARTICIPANT ADVOCATE More InfoThe Research Participant Advocate (RPA) is available to assist in protecting the safety and confidentiality of the research participants who volunteer for clinical studies. The RPA is a resource for potential research volunteers and a liaison and consultant to research teams.

The RPA can provide education and/or assistance on issues relating to human subject protection including the following:
Informed consent forms and processes
Data and safety monitoring plans and boards
Participant safety
Conflicts of interest
Regulatory statutes regarding human subjects
Adverse events
YesNo
Froedtert HospitalfhSLEEP LABORATORY More InfoA Sleep Laboratory, dedicated for clinical investigation, was recently established within the Adult Translational Research Unit (TRU) of the Clinical and Translational Science Institute (CTSI). Because of its portability, Sleep Lab equipment can also be used in any location outside the Adult TRU, including the intensive care unit and hospital wards. This new capability expands the number and types of studies that can be performed.YesNo
Froedtert HospitalfhTRANSLATIONAL CARDIAC AND VASCULAR FUNCTION UNIT More InfoThe Translational Cardiac and Vascular Function Unit, situated in the Adult TRU, has been folded into the TRUs for CTSI investigator use.
TECHNOLOGY & RESOURCES
GE Vivid 7 echocardiograph and 3S probe are available for human cardiac echocardiography
Data analyzed using GE Q analysis software (EchoPAC platform)
Three separate vascular ultrasound units (GE Logiq 500, Sonosite 180 plus, and a newer all digital Sonosite MicroMaxx unit, each with probes for high resolution vascular or cardiac imaging) are coupled with Brachial Analysis software for quantifying flow-induced vasodilation and with software for calculating intimal medial thickness and available exclusively for research
Separate Itamar EndoPat 2000 tonometry unit for non-invasive assessment of endothelial function is dedicated for use by CTSI investigators, a SphygmoCor® Px Pulse Wave Velocity System, and a SphygmoCor® Mx Pulse Wave Velocity System for non-invasive, reproducible measures of arterial stiffness has recently been acquired.
Hokanson Digital Venous Plethysmograph.
YesNo
Marquette UniversitymuBIOCHEMICAL LABORATORY More InfoThis is a full assay laboratory including point of care and standard equipment for biochemical assays of:

Lipids
Lactate
Glucose
Blood gases
Hemoglobin
Hematocrit
Salivary cortisols
DNA/RNA measurements
This laboratory also includes a full phlebotomy station.
NoNo
Marquette UniversitymuBIOINFORMATICS AND BIOMATHEMATICS LAB (BISTRO LAB) More InfoThe Bistro lab has housed several collaborations with faculty at the Medical College of Wisconsin since 2001. These include projects analyzing microarray data, analyzing documents curated by the Rat Genome Database, and analyzing full-text biomedical articles. Recently, new collaborations with faculty at the Department of Biological Sciences at Marquette University have been established to analyze the regulation and surveillance of non-coding RNAs.

Lab resources were also used in 2009 for drug candidate screening in collaboration with Dr. Daniel Sem at Marquette University and Dr. Brian Volkman at the Medical College of Wisconsin.

Currently, the lab is involved in analyzing high throughput genomic, genetic, and epigenetic datasets to model the gene regulatory networks in various organisms. The lab is also developing pipelines to analyze next-generation sequencing datasets from prostate cancer cell lines through collaboration with Dr. Bekir Cinar at UCLA. The lab is also developing techniques for analyzing large-scale copy number variant (CNV) data sets associated with congenital heart disease in collaboration with Dr. Aoy Mitchell at the Children’s Research Institute.

TECHNOLOGY & RESOURCES

Technical library
8 computer workstations
15 node cluster
Mac OS X based computers and configured for distributed and parallel processing tasks with Condor, providing 50 cores for computation
Over 5 terabytes (TB) of collective storage in the lab, which is used for storing major genomic data, a copy of MEDLINE for biomedical data mining, and other bioinformatics related data and tools
Bioinformatics software (e.g., EMBOSS, NCBI Tools, CLUSTALW, etc.)
Database software (MySQL, PostgreSQL)
Machine learning tools (e.g., Weka)
Statistical and mathematical software (R, MATLAB)
Document preparation software (LaTeX, OpenOffice, MS Office)
NoNo
Marquette UniversitymuBIOMECHANICS / MOTION ANALYSIS LABORATORY More InfoThis laboratory is used for teaching and research. The lab is equipment with Vicon MX systems the most advanced optical motion capture system available. Advanced undergraduate and doctoral courses utilize this state-of-the-art equipped laboratory for students to learn advanced techniques in biomechanical (kinematics and kinetics) and physiological assessments of human performance.

LOCATION

Cramer Hall 004B

TECHNOLOGY & RESOURCES

14 camera digital Vicon MX motion analysis system
16 channel Delsys Trigno wireless EMG/ accelerometer system
Two 600cm x 600cm AMTI fore platforms flush mounted in the floor
Biodex System 2 isokinetic dynamometer
Cosmed K4b2 portable CPET system
50” monitor display
Various Woodway treadmills
NoNo
Marquette UniversitymuBODY COMPOSITION LABORATORY More InfoEquipment available to investigators includes:

GE Lunar Prodigy DEXA
iDEXA
Digital C-arm Fluoroscope System
Analytical Scales
NoNo
Marquette UniversitymuCARDIAC RESEARCH FACILITY More InfoThe Cardiac Research Facility is an interdisciplinary research project between Dr. Fitts’ lab at MU and four MCW colleagues (Drs. Danny Riley, Dept. of Cell Biology & Anatomy, John Baker, Dept. of Surgery; Wai-Meng Kwok, Dept. of Anesthesiology; and Rohit Rao, Department of Medicine). The clinical aspects of the collaboration are to understand the cellular mechanisms by which pre-conditioning (via global ischemia) and programs of exercise-training protect against ischemic heart disease. A translational aspect is to investigate in the future how drug therapy plus exercise programs work synergistically to reduce the incidence of heart and respiratory muscle failure, and facilitate recovery from heart disease.

LOCATION

Department of Biological Sciences

TECHNOLOGY & RESOURCES

Langendorf perfusion apparatus (Fitts and Baker labs)
Electrophysiological myocyte station with microscope (Dr. Kwok)
Imaging center (Confocal microscope MU Biological Sciences Dept.)
EM facility (Dr. Riley and MCW)
Human Exercise testing center (Dr. Rao’s lab)
MU Exercise Science Dept. (in collaboration with Dr. Ng)
NoNo
Marquette UniversitymuDEPARTMENTS OF EXERCISE SCIENCE AND PHYSICAL THERAPY More InfoThe Departments of Exercise Science and Physical Therapy provide a collection of six labs for Neuromuscular Fatigue, Cardiovascular/ Neuromuscular function, Human Performance, Biomechanics/Ergonomics, Motion Analysis, and Neuromuscular Control of Movement.

TECHNOLOGY & RESOURCES

Biodex, multi- channel EMG
Electrical stimulation
Force transducers
Automated blood pressure monitors
Venous occlusion plethesmography
Shielded rooms for sympathetic microneurographic recordings
Motion analysis hardware and software
Two Treadmills
7 cycle ergometers
2 ECG + metabolic carts
Bioimpedance units
Aquisizer underwater treadmill
NoYes
Marquette UniversitymuDISCOVERY LEARNING CENTER More InfoThe Discovery Learning Center (DLC) offers machining and prototype shop services for mechanically-based student projects. It contains lathes, mills, and other equipment that faculty and students can use (after appropriate safety training) to construct prototypes or test fixtures and equipment. The DLC is located in the main engineering building and is accessible to all engineering faculty and students.

LOCATION

College of Engineering

TECHNOLOGY & RESOURCES

Lathe
HAAS TM-2 Mill
Prototyping
Truck and competition trailer
NoNo
Marquette UniversitymuEXERCISE PHYSIOLOGY LABORATORY More Info30 AUG EXERCISE PHYSIOLOGY LABORATORY
Posted at 14:09h in MU by Tina Taylor
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This lab is designed to evaluate both aerobic and cardiovascular parameters. Equipment available to investigators includes:

Treadmills (load capacity of 500 lbs running and 800 lbs walking for bariatric research)
2 Vmax Encore CPET systems
MetaMax 3b portable CPET system
2 Cardiac Assessment System for Exercise Testing (CASE) with treadmills
2 Marquette Electronics MAX-1 cardiac assessment systems with treadmills
Various electronically braked cycle ergometers
Zephyr Bioharness physiological monitoring system (telemetried HR monitoring for 25)
Z-Lift bodyweight supported treadmill system
4 Monark cycle ergometers
3 Monark upper body ergometers
4 recumbent cycle ergometers
Vascular Ultrasonography with AccessPoint software
NuStep
The laboratory is equipped with emergency medical equipment and full 12 lead and exercise lead configuration EKG.
NoNo
Marquette UniversitymuFALK NEUROREHABILITATION ENGINEERING RESEARCH CENTER (FNERC) More InfoThis 6,000 sq. ft center serves research and development (R&D) activities in neurorehabilitation, investigating intervention strategies for movement therapy. Integral to the FNERC are the Neurorobotic and Neuromotor Control Laboratories, the Neuromechanics Laboratory and the Neurocomputing and Modeling Laboratory. These labs offer numerous instruments for measuring and assessing human motor and sensory performance.

TECHNOLOGY & RESOURCES

30 foot walkway with a 7-camera Vicon motion capture system and embedded force plates within the floor
Experimental work stations including a custom wrist robot
Smartboard interface for visuomotor tests
Optotrak motion capture system for precision motion detection
NoNo
Marquette UniversitymuHI-INTENSITY X-RAY DIFFRACTOMETER MACROMOLECULAR CRYSTALLOGRAPHY More InfoThe laboratory works at the exciting interface of chemistry and biology, using the tools of protein engineering, kinetic analyses and X-ray crystallography to determine the structure and function of various enzyme systems at the molecular level. Structural and kinetic analyses, used in tandem, provide a powerful means to probe underlying mechanisms of disease and unveil new targets for therapeutic applications.

LOCATION

Wehr Life Sciences
NoYes
Marquette UniversitymuINTEGRATIVE NEUROSCIENCE RESEARCH CENTER More InfoThe INRC is serves to promote the exchange of ideas among Marquette neuroscience research faculty members, thereby increasing opportunities for collaborative research, acquisition of resources, attraction of high quality faculty and students and strengthening of educational offerings in this area.

LOCATION

Schroeder Health Sciences Complex 426

TECHNOLOGY & RESOURCES
Zeiss PASCAL laser confocal microscope
NoNo
Marquette UniversitymuMARQUETTE UNIVERSITY COMPUTATIONAL GRID (MUGRID) More InfoThe Marquette University computational grid (MUGrid) brings together a diverse team of investigators from mathematics, statistics, computer science, chemistry, electrical and computer engineering, mechanical engineering, and biomedical engineering to develop and utilize grid computing as an integral tool for computational research. This project expands the developing MUGrid with the acquisition of a new parallel computing cluster, storage and network infrastructure upgrades which will also provide the cyberinfrastructure for Marquette University’s new graduate programs in Computational Sciences.

The new parallel computing cluster and storage infrastructure will also be made available to the broader community through outreach activities. The resource will be part of Southeast Wisconsin High Performance computing consortium’s developing regional grid that will directly support academic/industry partnerships for healthcare, energy and water research in Southeast Wisconsin, and the most diverse region of the state

TECHNOLOGY & RESOURCES

Père, a 1024 core centralized compute cluster
A distributed Condor pool containing over 500 cores. Condor is software developed at UW Madison that scavenges idle cycles on computers and enlists them to do research
Pario, a 164 core cluster in the College of Engineering.
NoNo
Marquette UniversitymuMEDICAL IMAGING SYSTEMS LABORATORY More InfoThe Medical Imaging Systems Laboratory focuses on the design and optimization of medical imaging technologies, including CT and MR. Current research projects include investigating dose reduction, scatter reduction, image reconstruction and energy-weighting techniques for CT.NoNo
Marquette UniversitymuMOTION ANALYSIS/BIOMECHANICS LABORATORY More InfoRaptor-E Digital Real Time Camera Optical Capture System
Hawk Digital Portable Real Time Camera System
Animation Plugin Software
Talon Animation Plugin Software
Skeleton Builder, BioFeed Trak, KinTools RT and Calciun 10 Pack License, Cortex post processing licenses
Autodesk Education Suite for Entertainment Creation
Full Body Motion Capture Suits
Quad core processor tracking station
Cortex compatible laptop computer
NoNo
Marquette UniversitymuNIKON A1R CONFOCAL MICROSCOPY More InfoDepartment of Biological Sciences

TECHNOLOGY & RESOURCES

Nikon A1R Confocal Microscopy
NoNo
Marquette UniversitymuNMR FACILITY More InfoTECHNOLOGY & RESOURCES

Varian Mercury- 300
NMR System- 400
NMR System 600 MHz with a cryoprobe
Bruker Microflex MALDI-TOF MS
Shimadzu HPLC-MS 2020
Mattson and Nicolet Fourier transform infrared spectrometers
Two UV-Vis spectrometers
Fluorimeter
Polarimeter
HP GC-MS
Other smaller equipment
NoNo
Marquette UniversitymuORTHOPEDIC RESEARCH AND ENGINEERING CENTER (OREC) More InfoThe OREC partnership between MCW Departments of Orthopedic Surgery and Physical Medicine and Rehabilitation, MU Schools of Engineering and Dentistry, and Shriner’s Hospitals promotes and encourages advances in collaborative, translational, rehabilitation research and training. OREC supports scientific investigation in the areas of orthopedic biomechanics, human motion analysis, and rehabilitation engineering and includes three laboratories at MU and MCW.

These laboratories include the Human Motion Analysis Laboratory, a research and clinical facility that provides a controlled environment for assessment of ambulation, upper and lower extremity 3-D kinematics and kinetics, and extremity strength and motion testing, the Biomechanics Laboratory that supports basic science and applied research projects in orthopedic biomechanics, and the Biomaterials Research Lab which supports tissue engineering applicable to dentistry as well as orthopedics.

TECHNOLOGY & RESOURCES

Seven motion analysis cameras
Force plates
PC-based motion analysis work station
10-channel EMG system
Foot insole pressure measurement system
PCs for data analysis and motion analysis software
NoNo
Marquette UniversitymuSTRENGTH AND CONDITIONING RESEARCH LABORATORY More InfoThis lab is used to teach physical therapists, exercise physiologists and athletic trainers how to properly lift, design a program, safely spot or administer plyometrics. It may be utilized to strength train or for testing. The laboratory is equipped with:

Olympic Weight Lifting Equipment
Standard Clinical Resistance Training Equipment
600cm x 1200cm AMTI force platform flush mounted in an Olympic lifting platform
8 channel telemetered Delsys EMG system
4 camera Vicon Bonita motion analysis system
Brower Speed Trap II wireless timing system
Four 36” flat screen monitors
FreeMotion dual cable cross machine
Cybex Eagle Leg Press
Concept II rowing ergometer
Dumbbell set (1-100)
Olympic lifting platform
Magnum Fitness Smith Machine
Various Magnum Fitness resistance training equipment
Plyometric training equipment
Balance training equipment
Speed training equipment
Agility training equipment
NoNo
Marquette UniversitymuUBICOMP LAB More InfoThe Ubicomp Lab focuses on mHealth solutions, that is applications for mobile electronic devices, such as tablets or smart phones, to remotely measure, record, and transmit health data. The lab is currently providing customized mHealth solutions to a number of NIH R01, Healthier Wisconsin Partnership Program, and CTSI projects.

Currently the Ubicomp lab is involved in the development of:

Smart phone (iPhone, Android) / web / tablet (iPad, Samsung) versions of the available paper based tools in both multidisciplinary and community healthcare settings
Customized text messaging systems
Analog or digital sensor data collection on sensor boards (e.g. smart shoe, smart belt, smart watch, home robot) and sending the data to smart phones, HIPAA compliant cloud servers, or EMR
15+ sensor data collection systems from any smart phone
Reports for any device (smart phone, tablet, web) and conversion of the data to .csv files usable by any statistical package
Customized surveys (pre, post) on any device and the automatic email distribution of gift cards
Any customized real time assessment (e.g. EMA)
Data analysis algorithms and report generation
Emotion based solutions (e.g. pain management for palliative care)
Customized security and privacy solutions
Any futuristic smartphone and sensor based solutions for healthcare you can think of
TECHNOLOGY & RESOURCES

5 personal computers
4 servers
5 cloud server accounts
15 smart phones (iPhones, iPod touches, Android phones)
10 MacBook/laptop computers
NoNo
Marquette UniversitymuWATER QUALITY CENTER More InfoThe Water Quality Center brings together researchers, industry, government, private foundations and others to solve problems related to lake, river and groundwater quality. These problems often involve municipal wastewater, industrial wastewater and drinking water. Research is often multidisciplinary and is performed by experts from engineering, biological sciences and other disciplines. This 4000 sq. ft. lab facility in Marquette University’s Engineering Hall includes instrumentation for testing and applied research. Core Center members (and research speciality) includes Dr. Daniel Zitomer – Center Director (biotechnology for waste treatment and renewable energy generation; anaerobic biotechnology), Dr. Brooke Mayer (advanced oxidation processes; disinfection; virus inactivation; waste and water treatment) and Dr. Patrick McNamara (micropollutant fate and removal; triclosan, antibiotics and plasticizers fate and effects; biosolids management; wastewater treatment).

LOCATION

Engineering Hall, room 435

TECHNOLOGY & RESOURCES

Agilent 7500cx Inductively Coupled Plasma Mass Spectrometer
w/autosampler and computer control
BioRad DNA engine Thermal Cycler chassis and alpha units
Fotodyne Analyst Investigator workstation with filters and total lab
New Brunswick Innova 43 incubator shaker with platform
New Brunswick Model 2350 Open Air Shaker with platform
Bionumerics Software package
Brinkmann Model 3870E Compact Sterilizer
Labconco steamscrubber model 4400431, 230V
NoNo
Medical College of WisconsinmcwBIACORE 3000 INSTRUMENT More InfoThe BIAcore 3000 instrument integrates surface plasmon resonance (SPR) technology with a microfluidics system to monitor molecular interactions in real time at concentrations ranging from pM to mM. This label-free technology can detect a wide range of molecular masses from 180Da to >1000kDa. The high sensitivity and high through-put capabilities allows for the detection of drug-protein, hormone-protein, protein-protein, DNA-protein, carbohydrate-protein, and lipid-protein interactions. The ability to interface with mass spectrometers provides discovery-based research in proteomic studies.

The BIAcore 3000 instrument is available to all Medical College of Wisconsin faculty and staff who have been trained and demonstrate the ability to use microfluidic-based instrumentation. Training and consultation are available on an appointment basis.

LOCATION

Department of Biochemistry, BSB 367A
NoNo
Medical College of WisconsinmcwBIOENERGETICS SHARED RESOURCE More InfoThe MCW Cancer Center Bioenergetics Shared Resource (CCBSR) is dedicated to cancer research by utilizing cutting edge technology to make advancements in the field. The MCW CCBSR is located on the second floor in the MACC Fund Research Center (MFRC), Room 2013. Two main instruments are housed within the CCBSR laboratory: a Shimadzu Liquid Chromatography / Mass Spectrometry 8030 System (Shimadzu LC MS/MS (Fig. 2A) and a Seahorse Bioscience XF96 extracellular flux analyzer (Seahorse Bioscience XF96) (Fig. 2B). These instruments enable profiling and quantification of multiple metabolic pathways, including assessment of mitochondrial and glycolytic function in cancer cell culture models, allow for assessment of mitochondrial toxicity of novel chemotherapeutic agents, and can be used in longitudinal studies of tumorigenesis prevention. The Shimadzu LC MS/MS system is also capable of detecting and quantifying multiple metabolites simultaneously in biological samples. Investigators wishing to use these instruments design experiments in collaboration with the MCW CCBSR staff to ensure The MCW Cancer Center Bioenergetics Shared Resource (CCBSR) is dedicated to cancer research by utilizing cutting edge technology to make advancements in the field. The MCW CCBSR is located on the second floor in the MACC Fund Research Center (MFRC), Room 2013. Two main instruments are housed within the CCBSR laboratory: a Shimadzu Liquid Chromatography / Mass Spectrometry 8030 System (Shimadzu LC MS/MS (Fig. 2A) and a Seahorse Bioscience XF96 extracellular flux analyzer (Seahorse Bioscience XF96) (Fig. 2B). These instruments enable profiling and quantification of multiple metabolic pathways, including assessment of mitochondrial and glycolytic function in cancer cell culture models, allow for assessment of mitochondrial toxicity of novel chemotherapeutic agents, and can be used in longitudinal studies of tumorigenesis prevention. The Shimadzu LC MS/MS system is also capable of detecting and quantifying multiple metabolites simultaneously in biological samples. Investigators wishing to use these instruments design experiments in collaboration with the MCW CCBSR staff to ensure technical proficiency and accurate analysis of the data.

Services Provided: A wide range of experimental applications of extracellular flux and metabolomic studies can be performed with the instruments in the MCW CCBSR facility. Using the Seahorse Bioscience XF96 (a fluorescence-based microplate assay), we can simultaneously measure both oxygen consumption rate and extracellular acidification rate in a noninvasive manner. The Seahorse Bioscience XF96 system allows investigators to examine mitochondrial function, glycolytic function, fatty acid oxidation, glucose metabolism, glutamine metabolism, and metabolic phenotypes all in cancer models. Using the Shimadzu LC MS/MS, we offer enhanced and fast detection and quantification of extra- and intra-cellular substrates and metabolites, metabolic profiling/phenotyping, and drug/compound screening. The instruments are dedicated solely to analyze cancer bioenergetics, making the MCW CCBSR not only an important resource, but also extremely unique, since both instruments are not available elsewhere on the MCW campus.
NoNo
Medical College of WisconsinmcwBIOMOLECULAR NMR FACILITY More InfoThe NMR Facility is an interdepartmental research service unit located in the Biochemistry Department. High-field NMR spectroscopy is a powerful technique for the study of biomolecular structure and dynamics. The facility provides service for routine 1D and 2D NMR methods, and can also provide consultation and collaborative assistance with the acquisition and analysis of multidimensional, multinuclear protein NMR spectra. The facility operates two Bruker 600 MHz and one 500 MHz NMR spectrometers, each equipped with 1H/13C/15N cryoprobes for enhanced sensitivity in biomolecular applications. In addition, a Bruker 300 MHz NMR spectrometer is available for routine analytical NMR of small molecules. For some long-term projects, the facility provides training for instrument operation and data analysis to investigators and research personnel. The facility operates on a fee-for-service basis and is open to faculty of the Medical College of Wisconsin and outside researchers.

Equipment/Software
Two Bruker 600 MHz spectrometers with 1H/13C/15N cryoprobes
500 MHz NMR spectrometer with 1H/13C/15N cryoprobes
NoNo
Medical College of WisconsinmcwBIOPHOTONIC IMAGING CORE More InfoThe Biophotonic Imaging Core is available to all investigators for live animal imaging using bioluminescent or fluorescent molecules as reporters of gene or protein expression. Potential users must be trained on the instrument(s) and must have an IACUC-approved imaging protocol before using the core.

TECHNOLOGY & RESOURCES

IVIS Biophotonic™ Lumina
Maestro™ Multi-Spectral Imaging System
NoNo
Medical College of WisconsinmcwBIOSAFETY LEVEL 3 (BSL3) LABS AND ANIMAL BIOSAFETY LEVEL 3 (ABSL3) ROOMS More InfoThe BSL3 laboratories and ABSL3 rooms are core MCW facilities allowing manipulation and safe handling of pathogenic microorganisms requiring BSL3 and/or ABSL3 containment. This facility comprises approximately 10,000 square feet and includes dedicated BSL3 and ABSL3 suites. The BSL3 suite contains 4 separate containment labs, two of which are equipped for “enhanced” containment practices. Individual BSL3 laboratories range in size from approximately 130 square feet to 210 square feet and contain either one or two 6-ft class II-B1 cabinets.

The ABSL3 suite consists of 4 individual animal rooms. Each ABSL3 animal room is approximately 125 square feet and contains one 6-ft class II-B1 bio-safety cabinet and can house up one Allentown Bio-Containment rodent cage rack (Model: BCU-3000-30B), which holds 49 individually sealed and ventilated cages.

Equipment/Software
Class II-B1 biosafety cabinets
Allentown Bio-Containment Unit caging (Model: BCU-3000-30B)
Glas-Col Inhalation Exposure System
Coy Anaerobic Chamber
Flow cytometry instrument: Guava easyCyte HT
NoNo
Medical College of WisconsinmcwBIOSTATISTICAL CONSULTATION More InfoThe Biostatistics Consulting Service in the Division of Biostatistics at the Medical College of Wisconsin provides comprehensive statistical consulting and data entry services to clients inside and outside the Medical College.

Additionally, our faculty and staff have extensive experience in grant preparation of NIH, NSF, CTSI, Department of Education, HRSA, Healthier Wisconsin, and VA grants. We have also helped prepare proposals for a number of private research foundations.

LOCATION

Health Research Center, 2400

SERVICES

Assistance with design and analysis of clinical trials
Design and analysis of observational studies
Design and analysis of surveys
Assistance with public databases
Sample size and power calculations
Data analysis and interpretation
YesNo
Medical College of WisconsinmcwCARDIOVASCULAR CENTER (CVC) CORE EQUIPMENT More InfoThe CVC maintains an array of equipment for use by CVC members. Much of this equipment is located in core equipment rooms for which card access is required. Researchers in member labs who need access to specific pieces of equipment must apply to the CVC for access to the appropriate room(s) prior to requesting access from MCW. Some training may be required before access approval will be granted.

MCW faculty not directly associated with the CVC may request limited access by contacting Greg McQuestion.

Equipment/Software

BioRad Bio-Plex

Beckman DU640 spectrophotometer

Savant Speed-Vac

BioRad Gel Dryer

Nikon TE-2000U

Nikon E-55i

Nikon E600/spot RT

Microm cryostat

Typhoon imager – Out of Service

BioRad cell counter

BioRad iCycler real-time PCR

Nikon A1-R confocal Microscope

Surgical suite and animal prep (approved IACUC AUA# required) – CVC investigators only

Thermo Scientific RC-6 PLUS Superspeed Centrifuge

Beckman Coulter Optima XPN 100-IVD
NoNo
Medical College of WisconsinmcwCENTER FOR INFECTIOUS DISEASE RESEARCH SHARED RESOURCES More InfoThe mission of the Center for Infectious Disease Research (CIDR), formerly known as the Center for Biopreparedness and Infectious Disease (CBID), is to enhance research efforts that focus on understanding the molecular mechanisms of pathogenesis related to infection with all types of microorganisms, viruses, fungi or parasites. These efforts also include programs to define pathogen and host factors contributing to disease resistance or susceptibility, host recognition of foreign materials and the innate and adaptive immune responses following exposure to infectious organisms.

Equipment/Software

QP Expression Colony Picking Robot from Genetix

Gel documentation system: AlphaImager

Flow cytometry instrument: Guava easyCyte HT

Multicolor real-time PCR detection system:iCycler iQ

Ultrasonic liquid processor: Sonicator 3000

Suspension array system:Bio-Plex 200 system with Bio-Plex Pro II wash station

Ultracentrifuge:Optima TLX ultracentrifuge

French Press

Barnstead E-pur water system

BioRad CFX Real Time PCR system (owned by Coburn lab)

Nano Drop (owned by Zahrt lab)
NoNo
Medical College of WisconsinmcwCOMMUNITY TRU More InfoThe Community TRU helps accommodate research studies for larger, more long-term projects, incorporating flexibility as these locations change. Sites will be linked to the hospital-based TRUs for testing and procedures not available remotely or through the Mobile TRU.

The C-TRU establishes small, temporary research facilities throughout the Greater Milwaukee area. Facilities include underrepresented & minority community centers and agencies, as well as the Center for AIDS Intervention Research (CAIR) and similar cooperative centers.
YesNo
Medical College of WisconsinmcwELECTRON MICROSCOPY CORE FACILITY More InfoThe Electron Microscopy Core Facility at the Medical College of Wisconsin is an interdepartmental research service unit managed on behalf of the Medical College by the Department of Microbiology and Molecular Genetics and the Department of Cell Biology, Neurobiology and Anatomy. The facility provides service, consultation for research and some training for projects requiring transmission electron microscopy. The Facility operates on a fee-for-service basis and is open to all Medical College of Wisconsin faculty, staff, and students; investigators at affiliated institutions and other non-MCW investigators.

Services include:

Complete tissue processing facilities
Immuno-electron microscopy
Negative staining
Enzyme cytochemistry
Ultrastructural Electron Tomography

Equipment/Software
JEOL 2100 electron microscope equipped with a 2K x 2K ultrahigh resolution digital camera
Hitachi 600 electron microscope
Leica EMPact 2 high pressure freezing apparatus
Leica Automated Freeze Substitution apparatus
RMC Powertome & 6000 ultramicrotomes
NoNo
Medical College of WisconsinmcwEPIDEMIOLOGY DATA RESOURCE CENTER More InfoThe Epidemiology Data Resource Center (EDRC) is located within the Division of Epidemiology in the Institute for Health and Society and is a centralized resource for secondary health and demographic data and provides expertise in the use of spatial data and geographic information systems (GIS). Services include:

Generation of summary statistics
Preparation of dataset extracts
Lending data management and preparation expertise
Assistance with survey weighting and complex survey design analysis considerations
Assistance with long-term secondary data research projects
Mapping and other spatial analysis services
Resource for GIS information
Over 200 databases from various sources (for example, the Agency for Healthcare Research and Quality; Centers for Disease Control and Prevention; National Center for Health Statistics; US Census Bureau; Wisconsin Hospital Association/WI Bureau of Health Information; and more).

Equipment/Software
ArcGIS for Desktop
SAS
Stata
NoNo
Medical College of WisconsinmcwIRRADIATOR CORE More InfoThe Core consists of an irradiator that can be used to irradiate live animals, particularly mice and rats. One can also irradiate cells in culture using this instrument. Access to the irradiator requires training and approval by MCW radiation safety. Instrument-specific training will be conducted by the facility director, Dr. L. William Cashdollar.NoNo
Medical College of WisconsinmcwMACROMOLECULAR X-RAY CRYSTALLOGRAPHY FACILITY More InfoThe Department of Biochemistry houses state-of-the-art instrumentation dedicated to Structural Biology research. The facility includes chromatographic systems for protein purification, an in-house X-ray diffraction core and an automated crystallization system for high-throughput screening and optimization. High-end computer workstations have been set up for 3-D graphic visualization and crystallographic analysis.

The X-ray facility is located on the second floor of the Translational Biomedical Research Center (TBRC). The facility houses an X-ray diffraction system consisting of a Rigaku R-AXIS IV++ image plate detector system and MicroMax 007 generator equipped with Osmic confocal mirrors and an X-treme crystal cryo-cooler. The crystallization system includes a Hamilton STAR for solution making and a Phoenix for nanoliter crystallization and visualization.

The facility is open to faculty members of the Medical College of Wisconsin. Various levels of training are available and collaborative arrangements can be made to scientists both inside and outside of the MCW community.

Equipment/Software
Rigaku R-AXIS IV++ image plate detector system and MicroMax 007 generator equipped with Osmic confocal mirrors and an X-treme crystal cryo-cooler;

Hamilton Automated Crystallization System;

Phoenix Automated Crystallization System;

Graphics workstation
NoYes
Medical College of WisconsinmcwMASS SPECTROMETRY FOR PROTEOMICS – OFFICE OF RESEARCH More InfoMass Spectrometry Facility for Proteomics is a core laboratory within the Office of Research dedicated to the advancement of biomedical science through collaborative interactions with the research community at large. The Facility operates on a fee-for-service basis and specializes in protein identification, quantification, and characterization of post-translational modifications. Enzymatic digestion followed by sample purification is provided on a routine basis. Techniques for special sample handling include phosphopeptide enrichment, iTRAQ labeling, processing of SILAC-labeled samples, and sample fractionation. Bioinformatics tools are available for database searching and statistical analysis of complex data sets. Facility personnel work with investigators to help determine their experimental goals and to individually tailor the mass spectrometry workflow to suit their research needs.

LOCATION

Translational Biomedical Research Center, Room C0825

TECHNOLOGY & RESOURCES

Thermo LTQ Orbitrap Velos with ETD and Waters NanoAcuity HPLC*
Thermo LTQ Orbitrap Velos and Eksigent NanoLC 2D HPLC*
Thermo LTQ XL and Eksigent Ekspert 425 HPLC*
Max Quant software for database searching and Perseus software for post-search processing*
Mascot software for database searching*
Protein Center software for post-search processing*
Skyline software for experimental design and data analysis*
Thermo Xcalibur software for analysis of raw data*
Raw Meat software for assessment of raw data quality*
* Facility technicians use on behalf of investigators.
NoNo
Medical College of WisconsinmcwMRI SERVICES More InfoThrough the Center of Imaging Research, the CTSI Imaging Core unites basic and clinical scientists of various disciplines to further the development and application of functional imaging in health and disease. The CTSI Imaging Core provides cutting-edge imaging technology, expertise, and the state-of-the-art imaging facility to forge collaborative research among all eight CTSI partner institutions.

Equipment/Software
GE Healthcare MR750 3T Human MRI;

GE Healthcare MR950 7T Human MRI;

Bruker 9.4T Small Animal MRI;

Triumph Micro SPECT-CT;

CTSI Mock MRI Scanner for subject training (Froedtert East Pavilion)
NoNo
Medical College of WisconsinmcwOBSERVATIONAL METHODS More InfoThe Medical College of Wisconsin Cancer Center Observational Methods shared resource provides access to specialized services and expertise in the use of large databases. These datasets and the team’s knowledge support cancer research, grant applications, and publication in high-impact journals.

By providing access to specialized services and expertise, the Observational Methods Shared Resource is crucial not only to ensure the success of studies, but also to enhance the scientific interaction and productivity of researchers who perform observational database research.

Services & Resources:

Project feasibility and study design
Dataset availability
Preliminary data for grant applications
Basic data manipulation and analyses
New dataset identification and application process for purchase and acquisition
Database maintenance (provide server and data security measures)
Increase awareness of datasets and collaboration of researchers
Examples of Available Datasets:

Cancer-specific registries (National Cancer Database (NCDB), Surveillance Epidemiology and End Results (SEER), SEER-Medicare, state tumor registries)
Administrative databases (Chronic Condition Warehouse, Medicare)
Provider-related databases (American Medical Association’s Physician Professional database, American Hospital Association’s Annual Survey, Area Resource File)
Sociodemographic databases (Rural-Urban Commuting Area Codes (RUCA), U.S. Census Data, Area Resource File)
Also works closely with MCW’s Institute for Health and Society’s Epidemiology Data Resource Center and MCW’s Children’s Hospital’s Center for Clinical Effectiveness Research regarding additional available secondary datasets at this institution

Equipment/Software
Three servers (one 24 TB server and two 48X core application servers)
NoNo
Medical College of WisconsinmcwPATIENT CARE AND OUTCOMES RESEARCH (PCOR) INVESTIGATOR SUPPORT SERVICES More InfoThe MCW Center for Patient Care and Outcomes Research facilitates a supportive environment for MCW investigators developing funded research programs. Investigators work closely with administrative staff to plan and implement research programs as well as interface with faculty and programs throughout MCW. CME-accredited seminars occur on a biweekly basis to present and discuss research. Research groups formed around specific projects meet weekly to assess progress, discuss challenges and findings of ongoing work, and plan next steps. Biostatisticians affiliated with PCOR bring statistical expertise in several related areas including health services research, survey development and analyses, and scale development.

Services include:

Analysis of Complex Databases
Administrative databases (e.g., Medicare)
Clinical databases
Cancer registries
Linked clinical and administrative databases
Primary Data Collection
Qualitative research
Survey research
Prospective cohort studies
Intervention studies
Randomized trials of behavioral interventions
Community-Based Research
Community-based participatory research
Cross-cultural research methods
Development of Measures and Outcome Assessments
Quality of life measures
Survey development
Scale development
Knowledge Synthesis
Decision analysis and modeling
Cost-effectiveness
Meta-analyses / structured reviews
Comparative effectiveness

Equipment/Software
Hard drive storage and processing on dedicated servers
NoNo
Medical College of WisconsinmcwPHYSIOLOGY BIOCHEMICAL ANALYTICAL LABORATORY More InfoThe Physiological Biochemical Analytical Laboratory provides a consolidated, highly specialized, well equipped and professionally staffed analytical laboratory capable of performing a wide variety of immunoassays, HPLC based, clinical chemistry and biochemical analyses. The priority of this laboratory is to meet the analytical needs of members of the Department of Physiology and collaborating individuals.

Equipment/Software
Alfa-wasserman autoanalyzers optimized for clinical chemistry analysis on small samples obtained from rodents
6 fully automated HPLC systems equipped with UV
Fluorescent and absorbance plate readers
Fluorescent, radiochemical and electrochemical detectors
Beta and gamma counters
Centrifuges
Flame photometers
NoNo
Medical College of WisconsinmcwPRE-REVIEW SERVICE FOR NEW INVESTIGATORS More InfoCTSI’s Pre-Review Service for New Investigators provides services and information for all clinical and translational investigators. Its purpose is to provide assistance in research protocol, IRB submissions, conduct pre-reviews of regulatory documents, and support education regarding regulatory requirements.
FREE OF CHARGE, THIS SERVICE PROVIDES:
General information or brief consultation regarding availability of resources and services to support clinical and translational research
Education and training in meeting regulatory requirements for clinical research
Pre-review of investigator-prepared regulatory documents: a review of all draft documents prior to submission to the IRB to ensure that they are complete, correct, and consistent
Pre-review includes a comprehensive review of all draft documents to assure they are completed correctly and that the information presented is consistent among the study documents.
In addition, the protocol review includes analysis of recruitment procedures, data collection, risks, safety precautions, and protection of confidentiality. This review is done to assist investigators in presenting complete, accurate, and thorough applications prior to submission to the IRB.
Review is of full board, exempt, and expedited submissions.
A Pre-Review typically results in a significant decrease in the time required to attain final approval of the protocol
YesNo
Medical College of WisconsinmcwQUANTITATIVE MOLECULAR (CONFOCAL) IMAGING FACILITY More InfoThe Quantitative Molecular Imaging Facility is an interdepartmental research service unit located in the Biotechnology and Bioengineering Center. The facility includes a state-of-the-art Leica TCS SP5 Confocal Microscope System combined with fluorescence correlation spectroscopy (FCS) and multi-photon laser. We provide consultation and service for a variety of quantitative imaging of single molecules, cells, isolated tissues and organs, or intact animal preparations. The system is also capable of quantitatively analyzing molecular-molecular interactions in live cells using FCS as well as fluorescence energy transfer (FRET). Other specific features of the instrument include:

Ultra-high speed real time confocal imaging (250 frames/sec.)
Multi-photon laser scanning
Five channels detection with an Acousto-Optical Beam Splitter (AOBS)
Advanced multi-color restoration and spectral un-mixing capabilities
Fluorescence recovery after photo-bleaching (FRAP)
The facility will also provide training for operation and data analysis to investigators and research personnel. The facility operates on a fee-for-service basis and is open to faculty of the Medical College of Wisconsin and outside researchers.

Equipment/Software
Leica TCS SP5 Confocal Microscope System
Acousto-Optical Beam Splitter (AOBS)
NoNo
Medical College of WisconsinmcwRADIATION BIOLOGY SERVICE CENTER More InfoThe Service Center Irradiation Core is suitable for large animals, can irradiate small volumes of both large and small animals, and can irradiate tumors and cells at various dose rates. The Irradiation Core is available on a fee-for-service basis.

The service center also breeds, houses and sells the only WAG/Rij rats strain in the United States.

Equipment/Software
Teletherapy unit
X-RAD 320 kVp X-ray unit
Pantak 320 kVp X-ray unit
NoNo
Medical College of WisconsinmcwRAT RESEARCH MODELS SERVICE CENTER More InfoThe center provides the service of distributing specific rats (strain, line, chromosomes, and genetic codes). We ship animals within the facility as well as worldwide.NoNo
Medical College of WisconsinmcwREGENERATIVE MEDICINE CORE More InfoThe Regenerative Medicine Core seeks to encourage stem cell research at MCW by offering a laboratory (a) to guarantee the availability of ES cells that retain a normal karyotype and full pluripotency, (b) to maintain, characterize and transgenically manipulate human embryonic stem (ES) cells for investigational purposes, and (c) to offer training to investigators so that they can transfer the technologies to their own laboratories.

LOCATION

Basic Science Building-414

TECHNOLOGY & RESOURCES

3 normoxic and 3 hypoxic (4% O2) cell culture incubators (5% CO2)
Use is available only after training
5 tissue culture laminar flow hoods (Class 2 Type A, and A2 for lentivirus)
Use is available only after training
Nikon TMZ microscope for general use
Use is available only after training
PCR enclosure with Nikon SMZ800 stereomicroscope for manually selecting (scratching) pluripotent colonies
Use is available only after training
Nikon eclipse TE300 microscope configured for fluorescent imaging
Use is available only after training
BTX electroporation system
Use is available only after training
Amaxa Nucelofector II
Use is available only after training
Applied Biosystems StepOne Plus RT-PCR System
Use is available only after training
Applied Biosystems Proflex PCR System
Use is available only after training
SPOT imaging software ver 5.1.1
Use is available only after training
Environmental storage (limited)
Use is available only after training
Clinical (low speed) centrifuge
Use is available only after training
NoNo
Medical College of WisconsinmcwRESEARCH COMPUTING CENTER More Info
The Research Computing Center is a new unit within MCW IS dedicated to supporting researchers and receiving direction from the Office of Research. A faculty advisory group is being formed. The intent is to provide computer resources to MCW researchers at a heavily subsidized rate to enable better preliminary data analysis and enhance the resources available for meritorious applications.

Provide High Performance Computing Platforms

Manage open source scientific applications on three HPC platforms:

A 408 Core Linux MPI cluster
A 3 Terabyte RAM 40 Core Large Memory Linux server
Four Graphical Processing Unit Servers with Three Nvidia K40 (2880 core ) each
The current software list includes Charmm, Gromacs, NAMD, VMD, R, Bioconductor, Matlab, Bowtie2, Cufflinks, Hmmr, Tophat, Samtools, and FreeSurfer.

High Performance Storage

Provide large short term storage for analyses:

Isilon Storage pool 870TB with seven I/O nodes
Isilon Replication pool with 280TB with three I/O nodes
Tape Library with four LTO 6 (6.25 TB) drives for archiving
The Storage pool and the HPC Systems are connected by a dedicated 10Gb LAN for maximum throughput.

Software Licensing and Management

The RCC manages the license and license servers for EndNote, GraphPad Prism, X-Win32, Matlab, SPSS, PyMOL and AxPyMOL. Licenses are offered at a significant discount.

Research Server Management

The RCC will configure, install, manage and operate servers for Research Cores within the Office or Research.

Grant Submission Assistance

RCC will assist in evaluation of resources and processes needed to comply with data security requirements and provide boilerplate language to describe existing resources. It can also assist in identifying existing MCW resources that could be used for research efforts and design efficient solutions for research problems when needed.
NoNo
Medical College of WisconsinmcwRESEARCH EQUIPMENT SUPPORT AND ENGINEERING SERVICES (RESES) More InfoThe RESES Core is staffed by David Eick, a Mechanical Design Engineer with over 27 years’ experience in custom designing, building, and repairing of biomedical devices. The RESES Core can help researchers accomplish their goals while keeping costs at a minimum.

Services include:

Equipment repair
Calibration
Custom precision machinery
New equipment setup & technical consultation
Integrated solutions
Annual service contracts at competitive rates
Priority response available
NoNo
Medical College of WisconsinmcwSCIENTIFIC WRITING ASSISTANCE More InfoCTSI offers assistance to investigators writing research grant applications, manuscripts, patent applications, or other research-related materials.YesNo
Medical College of WisconsinmcwSEQUENCING SERVICE CORE More InfoThe HMGC Sequencing Service Core Facility is a state-of-the-art Next-Generation Sequencing fee-for-service facility serving the Medical campus as well as clients around the world. We can perform a broad range of Next-Generation and traditional sequencing applications and offer extremely high quality data at competitive prices.

Equipment/Software
Illumina HiSeq 2000/2500/MiSeq Sequencer
PacBio RS Sequencer
ABI 3730xl DNA Sequencer
Covaris E210 Acoustic DNA Shearer
Agilent Bioanalyzer
NoYes
Medical College of WisconsinmcwSHARED MASS SPECTROMETRY FACILITY More InfoThe MSMS Facility is an interdepartmental research service unit managed on behalf of the Medical College of Wisconsin by the Department of Pharmacology and Toxicology. The facility provides service and consultation for research projects requiring mass spectrometric analysis (fundamental, identification and quantitation) of a variety of compounds. The facility operates on a fee for service basis and is open for faculty of the Medical College of Wisconsin and outside researchers.

The MSMS Facility has various mass spectrometers with different configuration available for researchers’ needs in analysis of their samples.

Triple Quadrupole-LC Mass Spectrometer
The Agilent 6460 Triple Quad LC/MS/MS consists of electrospray ionization (ESI) source and triple quadrupole mass analyzer. The system is interfaced to a 1290 Infinity liquid chromatograph for fast chromatography. The mass analyzer has an upper range of 3000 m/z in both positive and negative detection modes and a data-sampling rate of 150 SRMs per second. The 6460 routinely achieves fmol sensitivity and it is suitable for the qualitative and quantitative determinations of small molecules such as lipids, steroids, drugs and metabolites, nucleic acids, and peptides. The fast data sampling allows rapid analysis of the most complex samples.

Triple Quadrupole-LC Mass Spectrometer
The Waters Quattro micro APCI mass spectrometer (LC/MS/MS) consists of Z-spray source for electrospray ionization (ESI) and chemical ionization (APCI) with the capability of performing a variety of CID experiments. The analyzer has a mass range up to 2000 m/z in both positive and negative detection modes. The Quattro is interfaced to a Waters Alliance 2695 liquid chromatograph and is used for qualitative and quantitative analysis of lipids, steroids, drugs and metabolites, nucleic acids, and peptides at pmol levels.

Triple Quadrupole-GC Mass Spectrometer
The ThermoElectron TSQ 8000 mass spectrometer (LC/MS/MS) is equipped for electron impact (EI) and chemical ionization (CI) with the capability of performing a variety of CID experiments. The analyzer has a mass range up to 1100 m/z in both positive, negative and pulsed positive-negative detection modes. The TSQ is interfaced to a Trace 1300 gas chromatography and is used for qualitative and quantitative analysis of smaller, more volatile lipids, steroids, drugs metabolites, and oxysterols at fmol to atmol levels.

Equipment/Software
Agilent 6460 Triple Quad ESI-LC/MS/MS
Waters Quattro micro API-LC/MS/MS
ThermoElectron TSQ 8000 –GC/MS/MS
NoNo
Medical College of WisconsinmcwSMALL ANIMAL ECHOCARDIOGRAPHY CORE More InfoThe Small Animal Echocardiography Core provides an imaging resource to support cardiovascular phenotyping efforts. The Core staff offers expertise, materials, training, consultation, access to equipment and performs scanning and analysis on a fee for service basis.

The Core serves Investigators affiliated with the Medical College of Wisconsin on a first come, first served basis. Time lines for projects vary according to the nature of the project and current workload of the Core. Information about fees is available from the Core Medical Director. Successful and timely animal scanning and data analysis requires a concerted, collaborative effort of the Investigator and the Core staff in all stages of the experiment. Investigators planning on utilizing Core services are therefore encouraged to contact the staff early in the planning stages of the experiment.
NoNo
Medical College of WisconsinmcwTISSUE BANK More InfoThe Medical College of Wisconsin Tissue Bank is a secure storage facility where blood and tissue samples are kept for use in future research. Currently, once a research study is approved, the researcher must spend a majority of their time collecting their own blood and tissue samples before they can begin their research.

The Medical College of Wisconsin Tissue Bank can improve this process by providing researchers with blood and tissue samples that have already been collected. This expedites the process so that research can begin quickly, and, most importantly, the possibility of discovering medications and possible cures for diseases can happen much sooner.

The MCW Tissue Bank offers the following services:

Plasma from whole blood
Buffy coat from whole blood
Fresh tissue procurement
Snap frozen tissue
OCT embedded tissue
Unstained slides
H&E stained slides (from frozen tissue)
Slide scanning
Frozen tissue QC
DNA from blood or tissue
RNA from blood or tissue
Satellite banking of specimens
Storage of specimens
If there is interest in services not listed above, the MCW Tissue Bank staff will work with investigators to determine feasibility of additional services whenever possible.

Equipment/Software
-80° upright freezers
Automated DNA/RNA isolation unit
Nanozoomer (slide scanner)
Slide and block storage cabinets
Tissue procurement gross bench
Biological cabinet
Centrifuges
Autostainer
OnCore BSM software
Liquid nitrogen
NoNo
Medical College of WisconsinmcwTRANSGENIC CORE More InfoThe Transgenic Core consists of a laboratory for procedures involving production and maintenance of transgenic mouse strains, a tissue culture facility for all procedures involving embryonic stem (ES) cells and gene targeting experiments, and a transgenic barrier holding facility. The Core is under the direction of Dr. Hartmut Weiler, who has extensive experience in the production of genetically altered mice, and includes three additional staff skilled in various aspects of transgenic animal production and related procedures.

Services

Transgenic mouse production by microinjection of oocytes
Gene targeting in ES Cells
Generation of ES cell chimeras
Sperm cryopreservation/recovery
Rederivation of mouse strains to achieve pathogen-free status
Injections of Crispr/Cas9 and Zinc Finger Nucleases

Equipment/Software
Microinjection Station
Dissecting Microscope w/color video capture
Microforge for production of customized needles/pipettes
Controlled rate Freezer for cryopreservation of cells and embryos
Gene Pulser
Needle Puller Fully automated
Teaching Stereoscope for microsurgery
Dissection Video Capture
Stero Zoom Microscope
NoNo
Milwaukee School of EngineeringmsoeBIOMEDICAL ENGINEERING CORE LABORATORIES More InfoThe biomedical engineering curriculum at MSOE is comprised of courses that develop skills in the areas of engineering, life sciences, mathematics, signal processing, medical imaging, systems physiology and modeling. Teamwork is stressed through a four-year design project in which students collaborate on a project related to some aspect of biomedical engineering. In the end, the reward is being fully prepared a career biomedical engineering.

LOCATION

1025 N Broadway

TECHNOLOGY & RESOURCES

Electronics design laboratories
Biomedical systems design laboratories
Physiology laboratory with twelve physiological parameter acquisition stations
Fully functional four bed ICU learning laboratory for BE and nursing students
Biomechanics laboratory
SERVICES

Education on the proper use of medical equipment
Consulting on the design of medical equipment
Design support to meet FDA requirements
Forensic analysis of medical equipment
NoNo
Milwaukee School of EngineeringmsoeBIOMOLECULAR ENGINEERING PROGRAM More InfoBiomolecular engineering is a diverse, application-driven discipline in the areas of medical, agricultural, environmental, biotechnological and other life-sciences fields. Globally, chemical, biological and nano industries seek engineering graduates who are prepared to work with the life sciences at a molecular level. Biomolecular engineering (BioE), a four-year Bachelor of Science degree program at MSOE, meets this need. The curriculum of the BioE program is a balanced combination of application-driven engineering, science, computational and biotechnology courses. Along with laboratory and rigorous math and science training, these courses address the cross-disciplinary nature of biomolecular engineering.

LOCATION

1025 N Broadway

TECHNOLOGY & RESOURCES

Wet Biotechnology Lab
Senior Design Lab
Dry Instrumentation Lab
BSL-II Cell Culture facility
NoNo
Milwaukee School of EngineeringmsoeCENTER FOR BIOMOLECULAR MODELING More InfoThe Center for BioMolecular Modeling creates unique physical models of molecular structures using rapid prototyping technologies. The center works with research scientists to create custom models of the proteins whose structures they are investigating. The center is unique in the world, bringing together the disciplines of engineering, structural biology and computer visualization.

LOCATION

1025 N. Broadway

TECHNOLOGY & RESOURCES

BioMolecular Modeling (CBM)
3D Molecular Designs (3DMD)
MSOE Walter Schroeder Library
NoYes
Milwaukee School of EngineeringmsoeENGINEERING RESEARCH CENTER FOR COMPACT AND EFFICIENT FLUID POWER More InfoThe Engineering Research Center for Compact and Efficient Fluid Power has been formally established through a multimillion dollar National Science Foundation award with strong support by the National Fluid Power Association. Each year, two graduate and two undergraduate students will be supported in addition to an REU student and a high school teacher. Strong interaction with our Rapid Prototyping Center and the new NanoEngineering Laboratory are anticipated.

MSOE is one of seven universities nationwide that is part of this research center for fluid power. MSOE’s involvement in the center will amplify its already significant work in fluid power, applying new technologies to the field. The lead university is the University of Minnesota. MSOE was chosen to be a part of this center because of its 40-plus year history in fluid power education and research, through its Fluid Power Institute™.

LOCATION

1025 N Broadway

TECHNOLOGY & RESOURCES

Rapid prototyping
Sensors
Nanotechnology
Tribology plus contamination analysis in fluid power to advance understanding of these areas, then help take them to the marketplace
NoNo
Milwaukee School of EngineeringmsoeFLUID POWER INSTITUTE™ More InfoThe Fluid Power Institute™ (FPI) at Milwaukee School of Engineering (MSOE) is one of the leading academic fluid power research laboratories in the nation. Established in 1962 the Fluid Power Institute laboratory is a leader in motion control and fluid power education, research and evaluation.

LOCATION

1025 N Broadway

TECHNOLOGY & RESOURCES

Specialized equipment for pump, valve, motor, cylinder and filter testing
FPI conducts endurance testing
Performance testing and environmental evaluations as well as component and system design, modeling and simulation
Reliability analyses and system integration
Fully instrumented Tribology laboratory is on hand to support research and conduct separate analysis projects
Capacity and horsepower to operate a wide variety of hydraulic components under high-pressure and flow conditions
NoNo
Milwaukee School of EngineeringmsoeNANOENGINEERING LABORATORY More InfoResearch and education at the nano-scale is becoming more critical each year as research and development focuses on nano-scale phenomena, ultrafine structures and interfaces between matter. Atomic Force Microscopy (“AFM”) allows the force between a small tip and a chosen sample surface to be measured with atomic-scale resolution. Initially lateral forces between the tip and the sample can also be measured to better understand the origins of friction at the molecular scale.

LOCATION

1025 N Broadway

TECHNOLOGY & RESOURCES

AFM surface measurement models
Plastic deformations
Electrical conductivity
Thermal conductivity
NoNo
Milwaukee School of EngineeringmsoePHOTONICS AND APPLIED OPTICS CENTER More InfoPhotonics and Applied Optics Center’s laboratories are in an extremely low-vibration site that allows performance of the most sensitive optical projects and experiments.

LOCATION

1025 N Broadway

TECHNOLOGY & RESOURCES

Six 4-by-8 foot optical tables
Picowatt optical power meters
Computer-controlled monochromators
Broad array of optical sources, including an optical time-domain reflectometer
NoNo
Milwaukee School of EngineeringmsoeRAPID PROTOTYPING CENTER More InfoMSOE’s Rapid Prototyping Center is a collective effort between education and industry. It serves as the technical hub for diverse manufacturing companies and educational organizations. Applications research involves industrial designs, process control, innovative materials, processing and structures, medical models and architectural uses. The RPC uses computer-based manufacturing techniques and complementary processes to reduce the time and cost of industrial products ranging from functional models to full-scale production. Four major AM technologies are available with seven installed systems: Binder jetting (3D Systems & ExOne), Material extrusion (Stratasys), Powder bed fusion (3D Systems) and Vat photo-polymerization (3D Systems). A 3D scanner is available along with software to convert CT or MRI data to CAD files.

LOCATION

820 N Milwaukee St
Milwaukee, WI 53202

TECHNOLOGY & RESOURCES

CMP-Hybrid advanced casting system
Nano-Engineering Laboratory
PlatyScan system
Altair Optistruct
Comsol
SolidWorks & ProEngineer
LabView
RP-Magics
NoNo
University of Wisconsin-MilwaukeeuwmAVI HIGH PERFORMANCE COMPUTING CLUSTER More InfoThe UWM High Performance Computing (HPC) Service provides powerful computational resources to UWM researchers and their student assistants from the College of Engineering and Applied Science, College of Letters and Science, School of Freshwater Sciences, and the College of Health Sciences. Each of the participating disciplines has its own cyberinfrastructure facilitator who aids researchers in getting started and making effective use of the service. Facilitators can also direct researchers to other resources that may be more applicable to their research.

LOCATION

University Information Technology Systems

TECHNOLOGY & RESOURCES

High performance computing cluster consisting of 142 Nehalem 5550 nodes (1,136 hyperthreaded cores)
24 gigabytes of memory per node and a high-throughput
Low-latency Infiniband network
SERVICES

Services include parallel and batch serial computing cycles with a variety of pre-installed software packages and the ability to add other applications and research developed codes.
NoNo
University of Wisconsin-MilwaukeeuwmBIOMEDICAL AND HEALTH TERMINOLOGY, ONTOLOGY, AND METADATA CORE More InfoCollection of terminology, ontology, and metadata resources;
Access to Unified Medical Language System Metathesaurus;
Access to Dictionary of American Regional English (D.A.R.E.);
Library of online learning modules;
SERVICES

Searching and browsing of terminology, ontology, and metadata resources
Consultation on the standards based design and construction of new
terminologies, ontologies, and metadata schema
Consultation on standards based design and construction of data dictionaries
LOCATION

Department of Health Informatics and Administration
College of Health Sciences
Northwest Quadrant B, Room 6536
2025 E. Newport Ave
Milwaukee, WI 53211-2906
NoNo
University of Wisconsin-MilwaukeeuwmCENTER FOR BIOMEDICAL DATA AND LANGUAGE PROCESSING More InfoThe mission of the Center for Biomedical Data and Language Processing (BioDLP) in the College of Health Sciences is to foster collaborative research towards the automatic processing of biomedical data. Research in the center aims to systematically integrate biomedical language processing, biomedical image processing, ontology/metadata development, and bioinformatics on omic data to facilitate information needs in the biomedical, clinical and biological community. Owing to the inherently collaborative nature of the research, the center strives to facilitate strong collaborations that include computer scientists, informaticians, linguists, and biomedical domain experts, like bio-curators and practicing clinicians.NoNo
University of Wisconsin-MilwaukeeuwmPEDIATRIC NEUROMOTOR LABORATORY More InfoThe Pediatric Neuromotor Laboratory is engaged in translational research, blending developmental inquiry with clinical application. Our emphasis is on skill acquisition and early motor control in infants and toddlers. Populations studied include: Spina Bifida, Down syndrome, and typical development. Undergraduate and graduate students contribute to the laboratory team.

LOCATION

College of Health Sciences
Pavilion, PT Suite

TECHNOLOGY & RESOURCES

6 camera motion analysis
16-channel sEMG
Infant treadmills
Pediatric motor test kits
MatLab/LabView software
Statistical packages
Toddler trikes (adapted and general)
NoNo
University of Wisconsin-MilwaukeeuwmREHABILITATION RESEARCH DESIGN AND DISABILITY (R2D2) CENTER More InfoThe R2D2 Center performs interdisciplinary basic research investigations, applied research and development, and innovative instruction related to technology and disability.

LOCATION

College of Health Sciences
Enderis Hall, Suite 135

TECHNOLOGY & RESOURCES

TUSK Kits, ADA-CAT
Eiki LC-XIP2000 Projector
Da-Lite 40449 Fast Folding Portable Rear Projection Screen
Access to various assistive technology tools
SERVICES

Building and space accessibility audits
Universal design consulting
Graphic design
Digital arts
Taxonomy development
NoNo
Zablocki VA Medical CenterzvamcBIOMECHANICS CORE More InfoMultiple specialty laboratories serve as “Biomechanics Research Core” facilities. Labs include electrohydraulic pistons and a separate bending-moment apparatus that apply simple or complex loads to biological or material specimens of various sizes. Custom-designed equipment to deliver blunt or blast brain injury to rodents and tissues are available. Additional devices include drop tower, pendulum impactor, pneumatically-driven servo-sled accelerator, and full-vehicle crash lab. High-speed video and 3D motion capture capabilities compliment over 400 channels of data acquisition equipment.

TECHNOLOGY & RESOURCES

3D motion capture systems
A computational lab with multiple high-end computer work stations
Vertical Drop Tower apparatus
High-speed video cameras
High-rate data acquisition systems
Servo-sled accelerator to study occupant responses
Vehicle crashworthiness laboratory includes a 500 ft track and barriers to conduct full-scale vehicle crashes
Crash test dummies including the Hybrid-III and EuroSID dummies
Shock-wave tube for blast simulation on rodents
Shock-wave tube for blast simulation on cells and tissue
Behavioral laboratory, including Morris Water Maze; Elevated Plus Maze; Open Field Test; Barnes Maze; Rotarod assessments Facility technicians use on behalf of investigators
10″ diameter shock tube for PMHS testing
Split Hopkinson Pressure Bar
Rotational acceleration brain injury device for rodents
Motion-tracking software system
Finite element mechanics software
NoNo
Zablocki VA Medical CenterzvamcCONFOCAL MICROSCOPY CORE More InfoThe VA Medical Center houses a confocal microscope for fluorescent imaging of tissue specimens or cell cultures. Capabilities include 3D optical sectioning, time-lapse imaging, spectral imaging, and imaging stitching or scanning. It is open to all investigators affiliated with the VA Medical Center or partnering institutions on a collaborative basis, with training required for access and online scheduling.

Equipment/Software
Leica TCS SP8 Laser Scanning Microscope
NoNo
Zablocki VA Medical CenterzvamcCRYSTAL ID CORE More InfoThe Crystal ID Laboratory specializes in the identification of crystalline materials in biologic samples, focusing on crystal-associated diseases such as kidney stone disease, crystal-associated lung diseases, and crystal-induced arthritis.

Technology & Resources

High-resolution x-ray powder diffraction
Fourier transform infrared spectroscopy as analysis methods
Extensive library of standards that have been assembled for both XRD and FTIR during over 30 years of analyses
Analyze crystalline materials from animal models and from synthetic processes

Equipment/Software
Two FTIR (Fourier Transform Infrared Spectroscopy)
Four XRD (X-ray powder diffraction)
NoNo
Zablocki VA Medical CenterzvamcIMAGING STATION-PLATE READER CORE More InfoThe Imaging Station-Plate Reader Core offers quantitative imaging of luminescent, fluorescent and colorimetric labeled biomolecules in a variety of assay formats including electrophoresis gels, gel blots, plates, tissues and in-vivo assays. Users must be trained prior to instrument use and software is available for remote analysis of imaging files. Use of the Imaging Station-Plate Reader Core is on a collaborative basis with utilizing online calendars for scheduling.

Technology & Resources

Imaging Stations for white light photography; Chemiluminescence detection (Western blots); UV Illumination (Ethidium Bromide gels, Fluorescent dye labels):

Carestream Health IS4000
Apelgen OmegaLum G
Plate Readers for reading 384, 96, 12 and 6 well formats:

PowerWave XS: Variable wavelength UV/VIS detection
Synergy 4: Fluorescent and Luminescent detection

Equipment/Software

Carestream Health IS4000 Imaging Station
Apelgen OmegaLum G Imaging Station
PowerWave XS: Variable wavelength UV/VIS detection plate reader
Synergy 4: Fluorescent and Luminescent detection plate reader
NoNo
Zablocki VA Medical CenterzvamcKECK FUNCTIONAL IMAGING LABORATORY (KFIL) More InfoThe Keck Functional Imaging Laboratory (KFIL) is a facility involving collaborators from the Medical College of Wisconsin, Marquette University and Clement J. Zablocki VA Medical Center. The two primary components of the lab are the unique open-design micro-CT and micro-SPECT systems for studying small animals, excised organs and tissue specimens. The x-ray source is ~3 microns which also enables high-resolution dynamic (30 frames/sec) planar imaging. Principal investigators in the laboratory use the systems primarily for the study of pulmonary physiology but it is adaptable for imaging many different biological phenomena. The facility is located at the VA Medical Center and was built and maintained with support from the W. M. Keck Foundation.

The X-ray imaging system was designed for both dynamics planar X-ray angiography and micro-CT (see Figure 2). This provides our group with the flexibility to acquire data on mass transport phenomena as well as detailed volumetric morphology. In both modes, emphasis was placed on high spatial resolution, on the order of tens of microns. Specifically, the system can acquire high frame rate planar images and short scan time volumetric data, which require high temporal resolution and high data throughput in addition to relatively low noise, large dynamic range, and a precision x-ray source and detector. The instrumentation also allows for precise manipulation of the specimen and detector position relative to the X-ray source providing fine controlled magnification and accurate selected regional imaging for data collection in real time.
NoNo
Zablocki VA Medical CenterzvamcZABLOCKI VA MEDICAL CENTER TRANSLATIONAL RESEARCH UNIT More InfoThe Zablocki VA Medical Center Translational Research Unit (V-TRU) is a resource for clinical investigators working with both veterans and non-veterans. Located on the fifth floor of the Veteran’s Medical Center, the unit consists of three fully equipped exam rooms and a laboratory specimen processing room.

The V-TRU Team is available to assist with services such as nursing support, study coordination, consenting, lab services and processing, manual muscle testing, medication administration, filling out case report forms and IRB assistance. Research nurses are able to assist investigators with research specific needs across multiple disciplines. The V-TRU can also connect you to cores within the Veteran’s Medical Center, including the Biomechanics Research Core, the Crystal ID Laboratory, the Imaging Station Core and the Keck Animal Imaging Core.

The V-TRU is currently participating in multiple studies including the Million Veteran Program (MVP). The MVP is a national study that aims to enroll one million Veteran volunteers from across the country to study how genes affect health. Researchers will collect blood samples and health information from the participants to create the world’s largest medical database that will be used to answer research questions and lead to new treatments.

SERVICES - Nursing support and dedicated nursing time
Study coordination
Use of exam rooms and equipment
Laboratory sample preparation and processing
Data management and consultations
IRB asssistance
Access to VA national databases
YesNo

NIH Funding Acknowledgment: Important Reminder – Please acknowledge the NIH when publishing papers, patents, projects, and presentations resulting from the use of CTSI resources by including the NIH Funding Acknowledgement.

PARTNERS
Zablocki VA Medical CenterFroedtert HospitalMedical College of Wisconsin Childrens Hospital of WisconsinBloodCenter of WisconsinUniversity of Wisconsin - MilwaukeeMarquette UniversityMilwaukee School of Engineering