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Froedtert Hospital

Froedtert Hospital

LocationLocationNameDescriptionLinkCTSI Core/ServiceFree Core Access
LocationLocationNameDescriptionLink
Froedtert HospitalfhADULT ECHOCARDIOGRAPHY CORE LABORATORY More InfoThe Adult Echocardiography Core provides high-quality cardiovascular imaging along with quantitative and qualitative imaging analysis for cardiovascular clinical trials and investigator initiated protocols. The Core faculty and staff offers expertise, materials, consultation, performs scanning and analysis on a fee for service basis.

The Core serves Investigators affiliated with the Medical College of Wisconsin. We provide consultation on image acquisition and analysis based upon American Society of Echocardiography (ASE) recommendations for the performance and evaluation of a comprehensive transthoracic echocardiogram, use of echocardiography in clinical trials and employ the ASE Standards for Echocardiography Core Laboratories.

Successful and timely scanning and data analysis requires a concerted, collaborative effort of the Investigator and the Core staff in all stages of the study. Investigators planning on utilizing Core services are therefore encouraged to contact the staff early in the planning stages of the study.
YesNo
Froedtert HospitalfhADULT TRANSLATIONAL RESEARCH UNIT More InfoScientists at the Medical College of Wisconsin and at any of the CTSI partner institutions who have an approved protocol can utilize the TRU facilities. We provide support services for a diverse array of research studies, which involve pulmonary, cardiovascular, chronic kidney, neurological (Alzheimer’s), metabolic, sickle cell, genetics and rare diseases to include a few.

The Adult TRU is located in close proximity to the main entrance of the Pavilion building of Froedtert Hospital. The unit is able to provide a wide array of basic clinical research services including nursing support, dedicated nursing time, use of exam rooms and equipment, laboratory sample preparation and consultations. Special arrangements can be made for studies requiring TRU support in a hospital setting.

SERVICES - The A-TRU team is available to assist with a variety of services including : Research nursing support and dedicated nursing time, Laboratory sample preparation and processing, Assistance with inpatient studies, Glucose Analysis, and Noninvasive BP Monitoring.
YesNo
Froedtert HospitalfhBIONUTRITION AND BODY COMPOSITION LAB More InfoThe Clinical and Translational Science Institute’s Bionutrition and Body Composition Lab is based out of the Adult Translational Research Unit (ATRU) and provides services to researchers from all CTSI affiliated institutions and community organizations.

Researchers and community health initiatives find that body composition and nutrition play an important role in promoting health and the treatment of disease. Even if nutrition and body composition are not a primary focus, an estimate on body composition and nutrient intake is often desired due to the influence both have on human physiology and public health determinates.

OVERVIEW

The CTSI Bionutritionist assists with planning, developing, implementing, and evaluating the nutrition and body composition components of research protocols and community programs. The ATRU houses a metabolic kitchen dedicated to providing general or protocol specific meals. It also has a body composition lab where whole body DXA scans, anthropometric measurements, bioelectrical impedance analysis, and energy assessment take place.
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Froedtert HospitalfhCLINICAL AND TRANSLATIONAL RESEARCH CORE LAB (CTRL) HISTOLOGY More InfoThe Clinical and Translational Research Core Lab (CTRL) combines two major components in one lab: histopathology and molecular pathology. The CTRL provides basic histopathology, research immunohistochemistry, tissue microarray, laser capture microdissection, and molecular biology services to researchers at the Medical College of Wisconsin and within the surrounding academic community.

Histopathology services include: tissue processing, embedding, and cutting both paraffin-embedded and frozen tissue. The CTRL performs standard stains, immunohistochemistry, antibody workup, and in situ hybridization. Tissue microarrays are constructed using a Veridiam VT110C. Digital imaging includes the ACIS III (DAKO), Visiopharm, and Nanozoomer (Hamamatsu). Specialized software is available to image and analyze tissue microarrays, and to manage and store array data.

The histology activities are performed by an HT (ASCP) certified histology technologist with twenty years of histology experience.

Histology Core Lab Services

Gross description and analysis of tissue specimens
Gross dissection of specimens (MOPEC)
Slide printer with barcoding (ThermoSlidemate)
Cassette printer with barcoding (ThermoPrintmate)
Tissue processing (Sakura VIP6)
Tissue paraffin embedding (Sakura TEK6)
Tissue sectioning to make slides (Thermo HM 355S)
Frozen sections (Thermo HM 525)
Slide staining (Leica Autostainer)
Immunohistochemical analysis (DAKO Autostainer XL)
Immunofluorescence (Olympus BX61)
Special staining
Tissue microarray construction (Veridiam)
Quantitative image analysis (DAKO ACIS III)
Laser capture microscopy (Arcturus)
Pathological interpretation
Tumor Identification

Equipment/Software

MOPEC Gross dissection of specimens

ThermoSlidemate slide printer with barcoding

ThermoPrintmate cassette printer with barcoding

Sakura VIP6 tissue processing

Sakura TEK6 tissue paraffin embedding

Thermo HM 355S tissue sectioning to make slides

Thermo HM 525 frozen sections

Leica Autostainer slide staining

DAKO Autostainer XL immunohistochemical analysis

Olympus BX61 immunofluorescence

Veridiam Tissue microarray construction

DAKO ACIS III quantitative image analysis

Arcturus laser capture microscopy

Leica Cytovision Software for FISH Analysis
NoNo
Froedtert HospitalfhCLINICAL AND TRANSLATIONAL RESEARCH CORE LAB (CTRL) MOLECULAR SERVICES More InfoThe Clinical and Translational Research Core Lab (CTRL) combines two major components in one lab: histopathology and molecular pathology. The CTRL provides basic histopathology, research immunohistochemistry, tissue microarray, laser capture microdissection, and molecular biology services to researchers at the Medical College of Wisconsin and within the surrounding academic community.

Molecular Diagnostic Services

The CTRL offers clinical molecular diagnostic testing for routine patient care, clinical trials, and research. Molecular services include: nucleic acid extraction from tissues (fresh, frozen, blood, formalin fixed paraffin embedded), nucleic acid quantification, PCR, real time PCR, quantitative PCR, dideoxy sequencing, massively parallel sequencing, capillary electrophoresis, gel electrophoresis, digital electrophoresis, and microarray analysis (Agilent format).

Molecular Core Lab Services

Nucleic acid purification (Qiagen BioRobot EZ1)
Nucleic acid quantification (Nanodrop; Qbit)
Agilent Bioanalyzer
PCR (ABI Veriti)
Real time PCR (Lightcycler; ABI 7500)
Reverse transcriptase PCR
Quantitative PCR (Lightcycler; ABI 7500)
Dideoxy sequencing (ABI 3500)
Ion Torrent PGM
DNA fragment analysis (ABI 3500)
DNA microarray analysis (Innopsys Scanner)
Ozone-free working environment (SciGene)
Gene expression microarray analysis (Innopsys)
Gel electrophoresis (Agarose, acrylamide)
Fluorescence in situ hybridization (FISH)

Equipment/Software

Qiagen BioRobot EZ1 nucleic acid purification

Nanodrop; Qbit nucleic acid quantification

Agilent Bioanalyzer

ABI Veriti PCR

Lightcycler2.0

ABI 3500 DNA fragment analysis and dideoxy sequencing

Ion Torrent PGM

Innopsys Scanner DNA/RNA microarray analysis

SciGene ozone-free working environment

Agarose, acrylamide gel electrophoresis

ABI 7500 Quantitative PCR
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Froedtert Hospitalfhi2b2 COHORT DISCOVERY TOOL More InfoThe i2b2 Cohort Discovery Tool provides an easy to use, self-service way for the Medical College of Wisconsin researchers to query the i2b2 Clinical & Translational Research Informatics Data Warehouse (CTRI-CRDW) to answer the question “Does the i2b2 CTRI-CRDW contain a cohort of patients with these characteristics?”. Search criteria can include patient demographics, ICD coded diagnoses, ICD and CPT coded procedures, laboratory test results, inpatient pharmacy orders and text within clinical documents, including surgical pathology reports and radiology reports.

In response to a query, the Cohort Discovery Tool returns the approximate number of patients matching the search criteria. No patient identifiers or clinical data is revealed. Researchers can store their cohort searches online and later meet with staff from the Medical College of Wisconsin Clinical & Translational Research Informatics team to discuss how they might review and extract detailed clinical data on patient cohorts for research purposes. Release of data from the i2b2 CTRI-CRDW for research purposes requires Medical College of Wisconsin IRB approval.
YesNo
Froedtert HospitalfhIRB NAVIGATOR More InfoInvestigators that have received an award from CTSI and require IRB approval for their study can enjoy the assistance of personalized IRB navigation: guidance through eBridge prior to submission, track protocol’s progress as it moves through the review process and track and troubleshoot delays to promote successful completion.

The IRB Navigator is a research service offered to investigators who have received an award from CTSI and require IRB approval for their study. The IRB Navigator provides investigators with assistance through the approval process and helps to ensure the process runs smoothly. Prior to, during and after the application is submitted through eBridge, the IRB Navigator can monitor it and troubleshoot to help advance the application to a successful completion. If there are areas that need further explanation prior to the application going to the IRB committee, the IRB Navigator acts as a liaison between the investigator and the IRB Coordinator to assist in providing clarification so the appropriate information is presented to reviewers and committee chair. After a study is approved, the IRB Navigator can assist with amendments and potentially reportable events.
YesNo
Froedtert HospitalfhLYMPHOCYTE PROPAGATION LABORATORY (LPL) More InfoThe Medical College of Wisconsin Cancer Center Lymphocyte Propagation Laboratory (LPL) is part of the Blood and Marrow Transplantation Program of the Medical College of Wisconsin. This laboratory provides cellular therapy product processing for human clinical trials. The laboratory primarily focuses on cellular therapy to improve the outcome of blood and marrow transplantation and the use of immunotherapy to combat human cancer.

The laboratory is CAP accredited, CLIA certified and is accredited by the Foundation for the Accreditation of Cellular Therapy (FACT) for the testing and processing activities that are performed.

Services include:

Consultation during early and mid-phase development
Identification of acceptable reagent and material sources
Assistance to prepare for FDA pre-IND meeting
Scale-up and/or transition to clinical grade product
Assist with writing of the chemistry, manufacturing, and controls (CMC) section of IND Application
Preparation of FDA compliant SOPs, workforms/batch records, and reports for product preparation and testing
Assist with the selection and validation of assays to ensure product safety, purity, and potency during phase I and II.
Production of products for clinical use and product storage
Release testing
Shipment to external sites
Prep and delivery of products for infusion
Post infusion immune monitoring with Flow Cytometry Shared Resource

Equipment/Software

CliniMACS Cell Separation Device X 2

CliniMACS Prodigy Cell Culture and Separation Device

BD Canto II Flow Cytometer

Top Count

LN2 Controlled Rate Freezers and Storage Tanks

Cobe 2991 Cell Washers x 2

Biological Safety Cabinets

Inverted and standard Microscopes

CO2 Incubators with Hepa Filtration

Sterile Connecting Devices and tubing sealers

Table Top and Floor Model Centrifuges X 4
NoNo
Froedtert HospitalfhOFFICE OF CLINICAL RESEARCH & INNOVATIVE CARE COMPLIANCE (OCRICC) More InfoEven with IRB approval, a project requiring use of any FH resource cannot begin activities without FH final Administrative Approval granted though OCRICC.

Your project must be reviewed by and receive FH Administrative Approval through the FH Office of Clinical Research & Innovative Care Compliance (OCRICC) if you plan to conduct your research at any Froedtert Health (FH) System entity (Froedtert Hospital, Community Memorial Hospital, St. Joseph West Bend, or any of our clinics), and/or utilize any FH resource for your research. These resources include, but not limited to, the following:

Cinical services (i.e., radiology imaging)
Surgical/interventional services
Staff time
Equipment
Space (i.e., inpatient, FH clinics, FH ancillary services, etc.)
Drugs
Devices
Lab services (except for services provided by Dynacare that are set up as external clients)
Specimens, including “discard” tissue from any FH or OR procedure area, and/or
Patient Health Information (PHI)
If you are unsure whether the resources involved in your project are FH resources, or if you are unclear exactly what resources you will need for your project, please contact OCRICC prior to your IRB submission.

OCRICC recommends that you contact them no later than submission to the IRB.
NoNo
Froedtert HospitalfhREDCap More InfoREDCap is a mature, secure web application for building and managing online surveys and databases. While REDCap can be used to collect virtually any type of data, it is specifically geared to support data capture for research studies. The REDCap Consortium is composed of 1,436 active institutional partners in 90 countries who utilize and support REDCap in various ways.YesNo
Froedtert HospitalfhRESEARCH PARTICIPANT ADVOCATE More InfoThe Research Participant Advocate (RPA) is available to assist in protecting the safety and confidentiality of the research participants who volunteer for clinical studies. The RPA is a resource for potential research volunteers and a liaison and consultant to research teams.

The RPA can provide education and/or assistance on issues relating to human subject protection including the following:
Informed consent forms and processes
Data and safety monitoring plans and boards
Participant safety
Conflicts of interest
Regulatory statutes regarding human subjects
Adverse events
YesNo
Froedtert HospitalfhSLEEP LABORATORY More InfoA Sleep Laboratory, dedicated for clinical investigation, was recently established within the Adult Translational Research Unit (TRU) of the Clinical and Translational Science Institute (CTSI). Because of its portability, Sleep Lab equipment can also be used in any location outside the Adult TRU, including the intensive care unit and hospital wards. This new capability expands the number and types of studies that can be performed.YesNo
Froedtert HospitalfhTRANSLATIONAL CARDIAC AND VASCULAR FUNCTION UNIT More InfoThe Translational Cardiac and Vascular Function Unit, situated in the Adult TRU, has been folded into the TRUs for CTSI investigator use.
TECHNOLOGY & RESOURCES
GE Vivid 7 echocardiograph and 3S probe are available for human cardiac echocardiography
Data analyzed using GE Q analysis software (EchoPAC platform)
Three separate vascular ultrasound units (GE Logiq 500, Sonosite 180 plus, and a newer all digital Sonosite MicroMaxx unit, each with probes for high resolution vascular or cardiac imaging) are coupled with Brachial Analysis software for quantifying flow-induced vasodilation and with software for calculating intimal medial thickness and available exclusively for research
Separate Itamar EndoPat 2000 tonometry unit for non-invasive assessment of endothelial function is dedicated for use by CTSI investigators, a SphygmoCor® Px Pulse Wave Velocity System, and a SphygmoCor® Mx Pulse Wave Velocity System for non-invasive, reproducible measures of arterial stiffness has recently been acquired.
Hokanson Digital Venous Plethysmograph.
YesNo

Important Note to Research Teams

Even with IRB approval, a project requiring use of any FH resource cannot begin activities without FH final Administrative Approval granted though OCRICC.

Your project must be reviewed by and receive FH Administrative Approval through the FH Office of Clinical Research & Innovative Care Compliance (OCRICC) if you plan to conduct your research at any Froedtert Health (FH) System entity (Froedtert Hospital, Community Memorial Hospital, St. Joseph West Bend, or any of our clinics), and/or utilize any FH resource for your research. These resources include, but not limited to, the following:

  • Cinical services (i.e., radiology imaging)
  • Surgical/interventional services
  • Staff time
  • Equipment
  • Space (i.e., inpatient, FH clinics, FH ancillary services, etc.)
  • Drugs
  • Devices
  • Lab services (except for services provided by Dynacare that are set up as external clients)
  • Specimens, including “discard” tissue from any FH or OR procedure area, and/or
  • Patient Health Information (PHI)

If you are unsure whether the resources involved in your project are FH resources, or if you are unclear exactly what resources you will need for your project, please contact OCRICC prior to your IRB submission.

OCRICC recommends that you contact them no later than submission to the IRB. You can contact them by email: ocricc@froedterthealth.org or their pages on the Froedtert & the Medical College of Wisconsin website.

NIH Funding Acknowledgment: Important Reminder – Please acknowledge the NIH when publishing papers, patents, projects, and presentations resulting from the use of CTSI resources by including the NIH Funding Acknowledgement.

PARTNERS

VersitiChildren's Hospital of WisconsinVA Medical CenterMarquette UniversityMSOEUWM