|Location||Location||Name||Description||Link||CTSI Core/Service||Free Core Access|
|Children's Hospital of Wisconsin||HISTOLOGY CORE More Info||The CRI Histology Core offers a broad range of high quality histological, immunohistochemical, and other related services on a fee-for-service basis to investigators from the Children’s Hospital of Wisconsin Research Institute, the Medical College of Wisconsin, and other institutions.|
This full-service Histology Core facility, operated in conjunction with the Pediatric Pathology Division of the Department of Pathology, offers internal and external users the following services:
Frozen and fixed tissue embedding
Cryostat and microtome sectioning
Routine H&E staining
Specialized histological staining
Immunohistochemical and immunofluorescent staining.
Optimization of immunostaining protocols using investigator-provided antibodies
Training in histological and immunohistochemical techniques
4 ASCP-certified histotechnologists and an American Board of Pathology-certified pathologist provide quality control.
Preparation of slides for laser capture microdissection
Preparation of tissue microarrays (in collaboration with Pediatric Biobank staff)
Close coordination of staining with imaging services provided by the physically adjacent CRI Imaging Core
Two Immunohistochemistry automated stainers (Leica – Bond Max Automated Stainer and DAKO Autostainer Plus)
Dako Artisan Special Stainer
Two Sakura Tissue-Tek VIP5 and one VIP6 tissue processors
Sakura Tissue-Tek Embedding Center Tec 5
Leica CM1850 UV Cryostat
Two Microm HM355S motorized rotary microtomes
Robotic routine stainers (Sakura Prisma)
Automated Coverslipper (Sakura Glas)
Leica IP S slide labeler
Leica Vibratome HM650 and Cooling Unit
|Children's Hospital of Wisconsin||IMAGING CORE More Info||We work with investigators at Children’s Hospital of Wisconsin Research Institute and other collaborating institutions to provide fee-based assisted and unassisted access to a variety of state-of-the-art microscopic imaging systems.|
Operated by the Pediatric Pathology Division at the Medical College of Wisconsin and Children’s Research Institute, our microscopy-based Imaging Core is open to all investigators at collaborating institutions.
Access to microscopic and imaging systems is controlled, monitored and protected by sign-up sheets, card reader entry and individualized computer account sign-on. Unassisted use of Imaging Core equipment requires prescribed training, demonstration of competency and approval by the core director. Investigators already affiliated with Children’s Research Institute can find more information about the Imaging Core’s equipment, fees and policies on the Children’s Hospital of Wisconsin intranet.
TECHNOLOGY & RESOURCES
Zeiss Axioimager Z1
Zeiss Axiovert 200M
Zeiss P.A.L.M. Microbeam III
Zeiss LSM510 confocal
Zeiss LSM510 META NLO multiphoton
|Children's Hospital of Wisconsin||MCW CANCER CENTER AND CHILDREN’S RESEARCH INSTITUTE (CRI) SHARED FACILITY More Info||The Medical College of Wisconsin Cancer Center (MCW-CC) and Children’s Research Institute (CRI) shared Flow Cytometry Facility is an advanced technology facility primarily serving investigators at the Medical College and the Children’s Research Institute. The facility provides secondary support to on-campus colleagues at the Blood Research Institute, as well as collaborators off campus at the University of Wisconsin-Milwaukee, Marquette University and the Marshfield Clinic.|
The first MCW-CC facility was established in 2000 with support from sources including a NIH Shared Instrumentation Grant, MCW Corporate Support, and the MCW-CC. The CRI facility was established in 2005 with support from the Children’s Foundation. In 2013, the two facilities merged in order to contain costs while improving access and service.
Now located on the 5th floor of the MACC Fund Research Center, the reorganized facility provides services to ~60 investigators campus-wide, for a combined total of more than 140 investigators served since 2000. The Children’s Research Institute and the Cancer Center share administrative oversight of the facility.
In addition to instrumentation, training, and cell sorting services, the facility also provides consultation to investigators regarding experiment design and protocol development based on instrument capabilities. The facility can advise antibody and fluorochrome selection based on targets of interest, appropriate compensation controls when needed, and provide software support for data analysis.
FACS Aria SOS
|Children's Hospital of Wisconsin||NUCLEIC ACID EXTRACTION CORE More Info||The Nucleic Acid Extraction Core provides investigators with fee-based services for DNA extraction using either manual and/or automated methods for the following sample types: Whole blood in small, medium or large volumes (400ul, 1-5mL, and 5-10mL); Tissue (fresh, frozen, and formalin fixed paraffin embedded tissue); and Buccal swabs.|
Children’s Research Institute, C4210
TECHNOLOGY & RESOURCES
Promega Maxwell 16
|Children's Hospital of Wisconsin||PEDIATRIC & ADOLESCENT MEDICINE TRANSLATIONAL RESEARCH UNIT More Info||Located on the fourth floor of Children’s Hospital of Wisconsin, the Pediatric Translational Research Unit (P-TRU) is a resource for all clinical investigators to perform the translational aspects of their behavioral, clinical and/or observation studies. The P-TRU services all ages from birth through geriatrics. Most of the research studies are specific to genetic congenital anomalies but range for all populations.|
The P-TRU team is available to assist with a variety of services:
Study management: from budgeting, planning, and regulatory to implementation
Our health unit coordinator, also a trained phlebotomist, is available to assist investigators with specimen processing
Friendly research nurses are available to assist investigators with research specific needs across multiple disciplines
Team members provide assistance with scattered bed placements, inpatient/outpatient research, behavioral health, interviews, lab services and qualitative health sciences
Grant development resources and assistance with protocol implementation are also provided
The P-TRU research team provides a unique combination of research and quality care while enhancing the quality of the experience for research participants
EPIC support for research specific eduation, order entry, and billing
The P-TRU offers five exam suites (two of which can accommodate large families) and also provides additional space for investigators to conduct interviews and obtain informed consent.
A -80˚ freezer
Pea Pod (which is mobile and allows for flexibility in location)
|Children's Hospital of Wisconsin||PEDIATRIC BIOBANK & ANALYTICAL TISSUE CORE More Info||The CRI Pediatric BioBank & Analytical Tissue Core, a research-supportive facility, directed by the Division of Pediatric Pathology with major support from the MACC Fund, is housed on the 4th floor of the TBRC/CRI building and provides quality-controlled banking of human pediatric tissue, blood, and urine samples for use by investigators at MCW and its affiliated institutions.|
Additionally, the core offers tissue analytical services, such as nucleic acid extraction (in collaboration with the CRI Nucleic Acid Extraction Core) from solid tissues (fresh, frozen, or formalin-fixed-paraffin-embedded) as well as from fresh or frozen blood, high-resolution digital microscope slide scanning, laser scanning cytometry, and image analysis for quantification of parameters in brightfield and fluorescent images.
Hamamatsu Nanozoomer slide scanner
Laser scanning cytometer
Visiopharm image analysis software
Promega Maxwell DNA extraction system
|Children's Hospital of Wisconsin||PEDIATRIC ECHOCARDIOGRAPHY CORE LABORATORY More Info||The Pediatric Echocardiography Core Lab housed in Children’s Hospital of Wisconsin was established in 2005 and has functioned as a resource for both internal and external projects that require performance of echocardiograms and/or analysis of echocardiographic data. The lab has received multiple NIH sub-contract grants from the Pediatric Heart Network (PHN) to act as an echo core lab for several large, multi-institutional trials: the Single Ventricle Reconstruction Trial, the Single Ventricle Reconstruction Extension Trial, and the Pediatric Echocardiography Database Z-score Project. The core lab will have analyzed over 7000 echocardiograms for these trials as well as collating and managing all of the echo data.|
In addition to the work with the NIH trials, the echo core lab has supported multiple industry and investigator-initiated research projects involving echocardiography. The echo core lab is located on the 2nd floor of the west tower of Children’s Hospital in the Herma Heart Center and provides services to investigators for both the adult and pediatric TRUs of the CTSI. We have trained staff with expertise in congenital heart disease and pediatric echocardiography (including 2D, 3D, and myocardial deformation imaging).
Children’s Hospital, Herma Heart Center
TomTec Workstations (3): HP Z420 with Xeon E5-1620 3.6 GHz (quad core), 10 MB cache per processor, 8 GB RAM, 500 GB hard drive, nVidia Quadro K2000 with 2 GB SDRAM video, DVD +/- RW drive with DVD RAM, Gigabit LAN networking, running Windows 7 64-bit operating system.
Image Arena TM 4.6 (Image analysis software – TomTec Imaging Systems, Germany): Diagnostic and report management system specifically designed for 2D/3D echo image review, archiving, and reporting with password-protected access
2D Cardiac Performance Analysis© software (ver. 126.96.36.199; TomTec Imaging Systems, Germany): Myocardial mechanics analysis tool that can analyze images from any US vendor (3 licensed PC’s)
PASW 18: (Predictive Analytic Software): Statistical Analysis Software
Sequoia 512 Ultrasound Machine (4, 8, and 10 MHz 2D imaging probes)
Accuson SC2000 Ultrasound Machine (8 and 4 MHz 2D imaging and 4 MHz 3D imaging probes)
Phillips IE33 Ultrasound Machine (12, 8, 5 MHz 2D imaging and 7 and 3 MHz 3D imaging probes)
Performance of 2D and 3D echo imaging studies (pediatric or adult)
Data collection tool development
Data collection and management
Technical image review and analysis
Investigator consults for planning and developing echo components of research protocols
Writing of echo components of research protocols, abstracts and manuscripts
Screening subjects for study eligibility
Data cleaning and basic descriptive statistical analysis*
*These services and fee schedules are discussed individually for each study
Echo image analysis training for students, nurses, research coordinators, residents, fellows and physicians.
NIH Funding Acknowledgment: Important Reminder – Please acknowledge the NIH when publishing papers, patents, projects, and presentations resulting from the use of CTSI resources by including the NIH Funding Acknowledgement.